A re-evaluation of diastatic Saccharomyces cerevisiae strains and their role in brewing

被引:26
作者
Krogerus, Kristoffer [1 ]
Gibson, Brian [1 ]
机构
[1] VTT Tech Res Ctr Finland Ltd, Tietotie 2,POB 1000, FI-02044 Espoo, Finland
关键词
Yeast; Beer; Dextrin; Starch; Diastatic; Genome; EXTRACELLULAR GLUCOAMYLASE; SIGNAL SEQUENCE; STA1; GENE; BETA-GALACTOSIDASE; MOLECULAR ANALYSIS; DIRECT CONVERSION; ENCODING GENE; ALPHA-AMYLASE; WILD YEAST; EXPRESSION;
D O I
10.1007/s00253-020-10531-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Diastatic strains of Saccharomyces cerevisiae possess the unique ability to hydrolyze and ferment long-chain oligosaccharides like dextrin and starch. They have long been regarded as important spoilage microbes in beer, but recent studies have inspired a re-evaluation of the significance of the group. Rather than being merely wild-yeast contaminants, they are highly specialized, domesticated yeasts belonging to a major brewing yeast lineage. In fact, many diastatic strains have unknowingly been used as production strains for decades. These yeasts are used in the production of traditional beer styles, like saison, but also show potential for creation of new beers with novel chemical and physical properties. Herein, we review results of the most recent studies and provide a detailed account of the structure, regulation, and functional role of the glucoamylase-encoding STA1 gene in relation to brewing and other fermentation industries. The state of the art in detecting diastatic yeast in the brewery is also summarized. In summary, these latest results highlight that having diastatic S. cerevisiae in your brewery is not necessarily a bad thing.
引用
收藏
页码:3745 / 3756
页数:12
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