Construction of core-shell tecto dendrimers based on supramolecular host-guest assembly for enhanced gene delivery

被引：44
作者：
Chen, Feng [1 ]
Kong, Lingdan [1 ]
Wang, Le [2 ]
Fan, Yu [1 ]
Shen, Mingwu [1 ]
Shi, Xiangyang [1 ]
机构：
[1] Donghua Univ, Coll Chem Chem Engn & Biotechnol, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China
[2] Shanghai Univ Engn Sci, Coll Chem & Chem Engn, Shanghai 201620, Peoples R China
来源：
JOURNAL OF MATERIALS CHEMISTRY B | 2017年 / 5卷 / 43期
基金：
中国国家自然科学基金;
关键词：
ENTRAPPED GOLD NANOPARTICLES; POLYMERS; CYCLODEXTRIN; CELLS; TRANSFECTION; THERAPY; TECTO(DENDRIMERS); NANOCARRIERS; ARCHITECTURE; DESIGN;
D O I：
10.1039/c7tb02585h
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
Design of dendrimer-based nanoarchitectures for enhanced gene delivery still remains a great challenge. Here, we report the design of core-shell tecto dendrimers using a supramolecular assembly approach for enhanced gene delivery applications. Firstly, beta-cyclodextrin (CD)-modified generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers (G5-CD) and adamantine (Ad)-modified generation 3 (G3) PAMAM dendrimers (G3-Ad) both having amine termini were synthesized. Through the supramolecular recognition of CD and Ad, G5-CD/Ad-G3 core-shell tecto dendrimers with a G5 core and G3 shell were formed. The formed G5-CD/Ad-G3 core-shell tecto dendrimers with a size of 8.4 nm possess good monodispersity, well-defined three-dimensional structure, and quite low cytotoxicity. Importantly, with the abundant amines on the surface, the core-shell tecto dendrimers are able to transfect the luciferase (Luc) gene with an efficiency 20 times and 170 times higher than the G5-CD and G3-Ad dendrimers, respectively. The higher gene transfection efficiency can also be qualitatively confirmed by transfection of plasmid DNA encoding enhanced green fluorescence protein. Our results suggest that the developed G5-CD/Ad-G3 core-shell tecto dendrimers may be used as a promising vehicle for enhanced gene transfection applications.
引用
收藏
页码：8459 / 8466
页数：8
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