Detection and characterization of Hepatitis E virus from commercial rabbit livers in Hebei, China

被引：1
作者：
Xiao Peng [1 ]
Tian Ji-jing [1 ]
Mao Jing-jing [1 ,2 ]
Guo Zhao-jie [1 ]
Zhao Yue [3 ]
Liu Tian-long [1 ]
Chen Jian [1 ]
Wang Tong-tong [1 ]
Ma Long-huan [1 ]
She Rui-ping [1 ]
机构：
[1] China Agr Univ, Coll Vet Med, Lab Anim Pathol & Publ Hlth Key Lab Zoonosis, Minist Agr & Rural Affairs, Beijing 100193, Peoples R China
[2] Shanghai InnoStar Biotech Co, Shanghai 201203, Peoples R China
[3] Jinan Univ, Inst Lab Anim Sci, Guangzhou 510632, Peoples R China
来源：
JOURNAL OF INTEGRATIVE AGRICULTURE | 2021年 / 20卷 / 12期
基金：
中国国家自然科学基金;
关键词：
hepatitis E virus; IHC; RT-nPCR; commercial rabbits; livers; LOCAL GROCERY STORES; FARMED RABBITS; IMMUNOHISTOCHEMISTRY; TRANSMISSION; INFECTION; GENOTYPE; HUMANS; JAPAN; SWINE; SOLD;
D O I：
10.1016/S2095-3119(21)63632-8
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Rabbit hepatitis E virus (HEV) has been reported for years and is thought to have the potential for zoonotic transmission from rabbits to humans. As reported, HEV genotype 3 (gt3) is the most prevalent form of HEV in rabbits. To determine the prevalence of HEV in commercial rabbit livers, 176 liver samples were collected from an abattoir in Hebei Province, China. Three (1.7%) samples tested positive for RNA of HEV-ORF2 (open reading frames-2). Sequence analysis showed that the three isolates shared high identities with each other (94.08-98.85%). Further analysis showed that all the rabbit strains clustered together in the branch of HEV gt3. Further study by immunohistochemistry (IHC) assays showed that 131 (74.4%) liver samples were positive for HEV ORF2 protein. Pathological changes including cell degeneration, inflammatory cell infiltration and bile duct epithelial cell hyperplasia were observed under microscopy. These findings indicated the presence of HEV in commercial livers of rabbits. Additional studies should be conducted to investigate the infectivity of rabbit HEV (rHEV) and the potential risks of zoonotic transmission of rHEV from rabbits to human beings.
引用
收藏
页码：3270 / 3276
页数：7
相关论文
共 37 条
[1]   Rabbit Hepatitis E Virus Infections in Humans, France [J].
Abravanel, Florence ;
Lhomme, Sebastien ;
El Costa, Hicham ;
Schvartz, Betoul ;
Peron, Jean-Marie ;
Kamar, Nassim ;
Izopet, Jacques .
EMERGING INFECTIOUS DISEASES, 2017, 23 (07) :1191-1193
[2]   Molecular virology of hepatitis E virus [J].
Ahmad, Imran ;
Holla, R. Prasida ;
Jameel, Shahid .
VIRUS RESEARCH, 2011, 161 (01) :47-58
[3]  
Ansari IH, 2000, J MED VIROL, V60, P275, DOI 10.1002/(SICI)1096-9071(200003)60:3<275::AID-JMV5>3.0.CO
[4]  
2-9
[5]   Detection of hepatitis E virus (rabbit genotype) in farmed rabbits entering the food chain [J].
Bigoraj, Ewelina ;
Kozyra, Iwona ;
Kwit, Ewa ;
Rzezutka, Artur .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2020, 319
[6]   Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands (vol 8, pg 227, 2016) [J].
Burt, Sara A. ;
Veltman, Jorg ;
Hakze-van der Honing, Renate ;
Schmitt, Heike ;
van der Poel, Wim H. M. .
FOOD AND ENVIRONMENTAL VIROLOGY, 2016, 8 (04) :313-313
[7]   Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands [J].
Burt, Sara A. ;
Veltman, Jorg ;
Hakze-van der Honing, Renate ;
Schmitt, Heike ;
van der Poel, Wim H. M. .
FOOD AND ENVIRONMENTAL VIROLOGY, 2016, 8 (03) :227-229
[8]   Molecular biology and pathogenesis of hepatitis E virus [J].
Chandra, Vivek ;
Taneja, Shikha ;
Kalia, Manjula ;
Jameel, Shahid .
JOURNAL OF BIOSCIENCES, 2008, 33 (04) :451-464
[9]   The ORF3 Protein of Hepatitis E Virus Delays Degradation of Activated Growth Factor Receptors by Interacting with CIN85 and Blocking Formation of the Cbl-CIN85 Complex [J].
Chandra, Vivek ;
Kalia, Manjula ;
Hajela, Krishnan ;
Jameel, Shahid .
JOURNAL OF VIROLOGY, 2010, 84 (08) :3857-3867
[10]   Hepatitis E Virus in Rabbits, Virginia, USA [J].
Cossaboom, Caitlin M. ;
Cordoba, Laura ;
Dryman, Barbara A. ;
Meng, Xiang-Jin .
EMERGING INFECTIOUS DISEASES, 2011, 17 (11) :2047-2049
1234