Single-shot phase contrast microscopy using polarisation-resolved differential phase contrast

被引：9

作者：

Kalita, Ranjan ^{[1
]}

Flanagan, William ^{[1
]}

Lightley, Jonathan ^{[1
]}

Kumar, Sunil ^{[1
,2
]}

Alexandrov, Yuriy ^{[1
,2
]}

Garcia, Edwin ^{[1
]}

Hintze, Mark ^{[3
]}

Barkoulas, Michalis ^{[3
]}

Dunsby, Chris ^{[1
,2
]}

French, Paul M. W. ^{[1
,2
]}

机构：

[1] Imperial Coll London, Phys Dept, Photon Grp, London SW7 2AZ, England

[2] Francis Crick Inst, London, England

[3] Imperial Coll London, Dept Life Sci, London, England

来源：

JOURNAL OF BIOPHOTONICS | 2021年 / 14卷 / 12期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国惠康基金; 英国工程与自然科学研究理事会;

关键词：

phase contrast microscopy; polarisation-resolved imaging; quantitative phase imaging; HIGH-RESOLUTION;

D O I：

10.1002/jbio.202100144

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a robust, low-cost single-shot implementation of differential phase microscopy utilising a polarisation-sensitive camera to simultaneously acquire four images from which phase contrast images can be calculated. This polarisation-resolved differential phase contrast (pDPC) microscopy technique can be easily integrated with fluorescence microscopy.

引用

页数：6

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