Estradiol control of expression and levels of estradiol-binding proteins in the medial preoptic area, medial hypothalamus and pituitary

被引:19
作者
Gao, G
Herbert, Z
Kong, J
Gabrielson, N
Mautz, A
Wu, DM
Jirikowski, GF
Caldwell, JD
机构
[1] Univ Illinois, Coll Med, Dept Biomed Sci, Rockford, IL 61107 USA
[2] S Dakota State Univ, Dept Pharmaceut Sci, Fargo, ND USA
[3] Univ Jena, Dept Anat, Jena, Germany
关键词
sex hormone-binding globulin; preoptic area; hippocampus; medial hypothalamus; paraventricular nucleus; neurohypophysis; gonadal steroids; gonadal steroid receptors; molecular neuroendocrinology; estradiol-binding protein;
D O I
10.1159/000071961
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The brains of mammals have at least three estradiol-binding proteins: estradiol receptor-alpha (ERalpha), ERbeta, and sex hormone-binding globulin (SHBG). In this study we compare the effects of estradiol treatment on the expression of mRNA for these three estradiol-binding proteins in two reproductively important brain areas, the medial preoptic area-anterior hypothalamus (MPOA-AH) and medial hypothalamus (MH) as well as in the hippocampus in ovariectomized rats, using the reverse transcriptase-polymerase chain reaction (RT-PCR). We also used surface-enhanced laser desorption ionization time of flight (SELDI-TOF) mass spectrometry (MS) to analyze the effects of estradiol in ovariectomized rats on SHBG levels in the MPOA-MH as well as the neurohypophysis. In vivo estradiol treatment in ovariectomized rats eliminated or significantly reduced expression of all three estradiol-binding proteins in both the MPOA-AH and MH. This change in ERalpha, ERbeta, and SHBG expression did not occur in the hippocampus. Both Northern blot and DNA sequence analysis confirmed the results of the RT-PCR for SHBG. SELDI-TOF MS analysis demonstrated that in vivo estradiol treatments resulted in dramatically decreased levels of SHBG in the hypothalamus and that a reduction in SHBG mRNA by estradiol treatment also resulted in a reduction in SHBG protein levels. Estradiol treatment also eliminated detectable SHBG from the neurohypophysis, suggesting that estradiol controls SHBG levels in this release site. That in vivo estradiol treatments had the same inhibitory effects on mRNA levels for SHBG and both ERs suggests similar translational control mechanisms for all three steroid-binding proteins in the brain. That estradiol treatments also reduced pituitary SHBG suggests that such treatment releases SHBG from the neurohypophysis. Copyright (C) 2003 S. Karger AG, Basel.
引用
收藏
页码:61 / 71
页数:11
相关论文
共 98 条
[1]   CELL TYPE-SPECIFIC AND REGION-SPECIFIC EXPRESSION OF AROMATASE MESSENGER-RNA IN CULTURED BRAIN-CELLS [J].
ABEDOHMAE, S ;
TANAKA, R ;
HARADA, N .
MOLECULAR BRAIN RESEARCH, 1994, 24 (1-4) :153-158
[2]   In vitro increase of aromatase mRNA in diencephalic neurons [J].
AbeDohmae, S ;
Tanaka, R ;
Takagi, Y ;
Harada, N .
NEUROENDOCRINOLOGY, 1996, 63 (01) :46-52
[3]   Distribution of immunoreactive androgen-binding protein sex hormone-binding globulin in tissues of the fetal rat [J].
Becchis, M ;
Sullivan, PM ;
Ordronneau, P ;
Petrusz, P ;
Joseph, DR .
STEROIDS, 1996, 61 (07) :392-400
[4]  
Caldwell J. D., 2001, Hormones and Behavior, V39, P326
[5]   MEDIAL PREOPTIC AREA OXYTOCIN AND FEMALE SEXUAL RECEPTIVITY [J].
CALDWELL, JD ;
JIRIKOWSKI, GF ;
GREER, ER ;
PEDERSEN, CA .
BEHAVIORAL NEUROSCIENCE, 1989, 103 (03) :655-+
[6]   5α-Reduced androgens block estradiol-BSA- stimulated release of oxytocin [J].
Caldwell, JD ;
Song, Y ;
Englöf, I ;
Höfle, S ;
Key, M ;
Morris, M .
BRAIN RESEARCH, 2003, 976 (02) :259-261
[7]   OXYTOCIN FACILITATES THE SEXUAL RECEPTIVITY OF ESTROGEN-TREATED FEMALE RATS [J].
CALDWELL, JD ;
PRANGE, AJ ;
PEDERSEN, CA .
NEUROPEPTIDES, 1986, 7 (02) :175-189
[8]   ESTROGEN INCREASES AFFINITY OF OXYTOCIN RECEPTORS IN THE MEDIAL PREOPTIC AREA ANTERIOR HYPOTHALAMUS [J].
CALDWELL, JD ;
WALKER, CH ;
PEDERSEN, CA ;
BARAKAT, AS ;
MASON, GA .
PEPTIDES, 1994, 15 (06) :1079-1084
[9]   Analogies between oxytocin systems of the uterus and brain [J].
Caldwell, JD ;
Walker, CH ;
ORourke, ST ;
Faggin, BM ;
Morris, M ;
Mason, GA .
HORMONE AND METABOLIC RESEARCH, 1996, 28 (02) :65-74
[10]   NOREPINEPHRINE INFUSIONS INTO THE MEDIAL PREOPTIC AREA INHIBIT LORDOSIS BEHAVIOR [J].
CALDWELL, JD ;
CLEMENS, LG .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 1986, 24 (04) :1015-1023