Clinical Evaluation of a New Quantitative Enzyme-Linked Immunosorbent Assay for Anti-dsDNA Antibodies Measured in Patients With Systemic Lupus Erythematosus

被引：0
作者：
Cui, Liyan [1 ]
Zhang, Jie [1 ]
Hu, Xiaozhou [1 ]
Zhang, Wenjing [1 ]
Ding, Jie [1 ]
机构：
[1] Peking Univ, Dept Lab Med, Hosp 3, Beijing 100871, Peoples R China
来源：
LABMEDICINE | 2010年 / 41卷 / 12期
关键词：
immunology; AP technique; AUTOANTIBODIES; SPECIFICITY; PREDICTORS;
D O I：
10.1309/LMLJNSSXX4PC37PL
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background The measurement of autoantibodies specific for double stranded DNA (anti dsDNA) is a useful tool for the diagnosis and prognosis of systemic lupus erythematosus (SLE) A quantitative enzyme linked immunosorbent assay (ELISA) for anti dsDNA antibodies has recently become available for the determination of anti dsDNA antibodies The aim of this study was to evaluate the clinical performance characteristics of this assay in patients with inactive or active SLE Methods Serum samples from patients with inactive (n=65) or active (n=112) SLE were tested for anti dsDNA autoantibodies using 2 methods from Euroimmun AG (Lubeck, Germany) a new ELISA anti dsDNA assay and the currently available Crithidia luciliae indirect immunofluorescent test (CLIFT) In addition, the levels of the complement components C3 and C4 biological markers associated with SLE disease activity were measured by nephelometry in the sera from all patients Results The sensitivity and specificity of the anti dsDNA ELISA assay for discriminating between patients with inactive or active SLE were 93 54% and 67 69% respectively Among all patients (n=177) there was a weak concordance (kappa=0 264) between anti dsDNA results by the ELISA and CLIFT assay However a significant inverse correlation was found with complement components levels In addition, area under the curve (AUC) values for anti dsDNA as well as C3 and C4 assays decreased in the following order 0 901 (ELISA for anti dsDNA autoantibodies)>0 734 (C3)>0 723 (C4)>0 650 (CLIFT for anti dsDNA autoantibodies) Conclusions Our results show the ELISA for anti dsDNA autoantibodies is significantly more sensitive and specific than any of the other assays we evaluated for discriminating between patients with inactive or active SLE Moreover, the inverse correlation between ELISA anti dsDNA autoantibody and complement levels suggests that anti dsDNA results by the Euroimmun ELISA may be promising for monitoring disease activity in patients with SLE
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页码：724 / 727
页数：4
相关论文
共 15 条
[1]   Development of autoantibodies before the clinical onset of systemic lupus erythematosus [J].
Arbuckle, MR ;
McClain, MT ;
Rubertone, MV ;
Scofield, RH ;
Dennis, GJ ;
James, JA ;
Harley, JB .
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 349 (16) :1526-1533
[2]   DERIVATION OF THE SLEDAI - A DISEASE-ACTIVITY INDEX FOR LUPUS PATIENTS [J].
BOMBARDIER, C ;
GLADMAN, DD ;
UROWITZ, MB ;
CARON, D ;
CHANG, CH .
ARTHRITIS AND RHEUMATISM, 1992, 35 (06) :630-640
[3]   THE SPECIFICITY OF THE ANTI-DSDNA ELISA - A CLOSER LOOK [J].
BRINKMAN, K ;
TERMAAT, R ;
VANDENBRINK, H ;
BERDEN, J ;
SMEENK, R .
JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 139 (01) :91-100
[4]  
CASALS MR, 2004, LUPUS, V13, P777
[5]   Laboratory tests as predictors of disease exacerbations in systemic lupus erythematosus - Why some tests fail [J].
Esdaile, JM ;
Abrahamowicz, M ;
Joseph, L ;
MacKenzie, T ;
Li, Y ;
Danoff, D .
ARTHRITIS AND RHEUMATISM, 1996, 39 (03) :370-378
[6]  
LEBLANC BAEW, 1994, J RHEUMATOL, V21, P2239
[7]   DEFINITION, INCIDENCE, AND CLINICAL DESCRIPTION OF FLARE IN SYSTEMIC LUPUS-ERYTHEMATOSUS - A PROSPECTIVE COHORT STUDY [J].
PETRI, M ;
GENOVESE, M ;
ENGLE, E ;
HOCHBERG, M .
ARTHRITIS AND RHEUMATISM, 1991, 34 (08) :937-944
[8]   Autoantibody explosion in systemic lupus erythematosus: More than 100 different antibodies found in SLE patients [J].
Sherer, Y ;
Gorstein, A ;
Fritzler, MJ ;
Shoenfeld, Y .
SEMINARS IN ARTHRITIS AND RHEUMATISM, 2004, 34 (02) :501-537
[9]  
Smeenk Ruud J. T., 1996, P227, DOI 10.1016/B978-044482383-0/50034-0
[10]   PREDICTIVE VALUE OF COMPLEMENT PROFILES AND ANTI-DSDNA IN SYSTEMIC LUPUS-ERYTHEMATOSUS [J].
SWAAK, AJG ;
GROENWOLD, J ;
BRONSVELD, W .
ANNALS OF THE RHEUMATIC DISEASES, 1986, 45 (05) :359-366
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