Synthesis of quinoline thioethers as novel small molecule enhancers of monoclonal antibody production in mammalian cell culture

被引：10
|
作者：
Kazi, Shahid A. [1 ]
Kelso, Geoffrey F. [1 ]
Harris, Simon [1 ]
Boysen, Reinhard I. [1 ]
Chowdhury, Jamil [1 ]
Hearn, Milton [1 ]
机构：
[1] Monash Univ, Ctr Green Chem, Melbourne, Vic 3800, Australia
来源：
TETRAHEDRON | 2010年 / 66卷 / 48期
基金：
澳大利亚研究理事会;
关键词：
Small molecule enhancer; Monoclonal antibody; Hybridoma; IgG; Quinoline thioether; IDENTIFICATION; BUTYRATE; ACID;
D O I：
10.1016/j.tet.2010.09.020
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A small library of novel quinoline derivatives containing different thioether substituents at position 4 have been synthesized and screened in murine hybridoma cell culture for their ability to enhance monoclonal antibody (mAb) production. From this set of compounds, four compounds have been discovered that enhanced immunoglobulin G (IgG) titer over negative control cultures due to an increase in specific productivity. These results demonstrate the utility of using organic synthesis and parallel ligand screening methods to discover novel cell culture additives that may be useful for increasing mAb yield in industrial biomanufacturing processes. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：9461 / 9467
页数：7
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