Mass-based readout for agglutination assays

被引：9

作者：

Chunara, Rumi

Godin, Michel

Knudsen, Scott M.

Manalis, Scott R. ^{[1
]}

机构：

[1] Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[2] MIT, Biol Engn Dept, Cambridge, MA 02139 USA

[3] MIT, Biol Engn & Mech Engn Dept, Cambridge, MA 02139 USA

来源：

APPLIED PHYSICS LETTERS | 2007年 / 91卷 / 19期

基金：

美国国家卫生研究院; 加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1063/1.2806197

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.

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页数：3

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