Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA

被引：25
作者：
Kumar, Pawan [1 ]
El-Sagheer, Afaf H. [1 ,2 ]
Truong, Lynda [1 ]
Brown, Tom [1 ]
机构：
[1] Univ Oxford, Dept Chem, 12 Mansfield Rd, Oxford OX1 3TA, England
[2] Suez Univ, Fac Petr & Min Engn, Dept Sci & Math, Chem Branch, Suez 43721, Egypt
来源：
CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 63期
基金：
英国生物技术与生命科学研究理事会;
关键词：
TERMINAL ALKYNES; OLIGONUCLEOTIDES; ANALOGS; AZIDES; BIOCOMPATIBILITY; CYCLOADDITION; STABILITY; CHEMISTRY; LINKAGES; LIGATION;
D O I：
10.1039/c7cc05159j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Oligonucleotides containing internal triazole-3'-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified oligonucleotides, and significantly better than oligonucleotides containing triazole alone. In contrast LNA on the 50-side of the triazole does not stabilise duplexes. Triazole-LNA confers great resistance towards enzymatic degradation relative to LNA alone.
引用
收藏
页码：8910 / 8913
页数：4
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