Macromolecular crowding: qualitative and semiquantitative successes, quantitative challenges

被引：390

作者：

Hall, D ^{[1
]}

Minton, AP ^{[1
]}

机构：

[1] NIDDKD, Sect Phys Biochem, Lab Biochem & Genet, Natl Inst Hlth,US Dept Hlth & Human Serv, Bethesda, MD 20892 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2003年 / 1649卷 / 02期

关键词：

macromolecular solution; excluded volume; thermodynamic nonideality;

D O I：

10.1016/S1570-9639(03)00167-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The concept of excluded volume and the theory of effects of excluded volume on the equilibria and rates of macromolecular reactions in fluid media containing high total concentrations of macromolecules ('crowded' media) are summarized. Reports of experimental studies of crowding effects published during the last year are tabulated. Limitations of current excluded volume theory are discussed, and a determination is made of conditions under which this theory may and may not be validly applied. Recently suggested novel approaches to quantitative analysis of crowding phenomena, which may help to overcome some of the limitations of current theory, are summarized. Published by Elsevier B.V.

引用

页码：127 / 139

页数：13

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