Measurement of Microvesicle Levels in Human Blood Using Flow Cytometry

被引：56

作者：

Chandler, Wayne L. ^{[1
,2
]}

机构：

[1] Seattle Childrens Hosp, Dept Labs, OC 8-734,4800 Sandpoint Way NE, Seattle, WA 98105 USA

[2] Univ Washington, Dept Lab Med, Seattle, WA 98195 USA

来源：

CYTOMETRY PART B-CLINICAL CYTOMETRY | 2016年 / 90卷 / 04期

关键词：

microvesicles; microparticles; flow cytometry; platelets; annexin V; CELL-DERIVED MICROPARTICLES; PLATELET MICROPARTICLES; CIRCULATING MICROPARTICLES; PROCOAGULANT ACTIVITY; PLASMA; CENTRIFUGATION; COINCIDENCE; CALIBRATION; HEMOSTASIS; ASSAY;

D O I：

10.1002/cyto.b.21343

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Microvesicles are fragments of cells released when the cells are activated, injured, or apoptotic. Analysis of microvesicle levels in blood has the potential to shed new light on the pathophysiology of many diseases. Flow cytometry is currently the only method that can simultaneously separate true lipid microvesicles from other microparticles in blood, determine the cell of origin and other microvesicle characteristics, and handle large numbers of clinical samples with a reasonable effort, but expanded use of flow cytometric measurement of microvesicle levels as a clinical and research tool requires improved, standardized assays. The goal of this review is to aid investigators in applying current best practices to microvesicle measurements. First pre-analytical factors are evaluated and data summarized for anticoagulant effects, sample transport and centrifugation. Next flow cytometer optimization is reviewed including interference from background in buffers and reagents, accurate microvesicle counting, swarm interference, and other types of coincidence errors, size calibration, and detection limits using light scattering, impedance and fluorescence. Finally current progress on method standardization is discussed and a summary of current best practices provided. (C) 2016 Clinical Cytometry Society

引用

页码：326 / 336

页数：11

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