Determination of 3-nitrotyrosine in human urine samples by surface plasmon resonance immunoassay

被引：19

作者：

Jin, Jing ^{[1
,2
]}

Wang, Chunyan ^{[3
]}

Tao, Yi ^{[1
]}

Tan, Yingjun ^{[3
]}

Yang, Dacan ^{[1
]}

Gu, Yin ^{[3
]}

Dengb, Huihua ^{[2
]}

Bai, Yanqiang ^{[3
]}

Lu, Hua ^{[1
]}

Wan, Yumin ^{[3
]}

Lu, Zuhong ^{[1
,2
]}

Li, Yinghui ^{[3
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, Chien Shiung Wu Lab, Nanjing 210096, Peoples R China

[2] Southeast Univ, Key Lab Child Dev & Learning Sci, Minist Educ, Nanjing 210096, Peoples R China

[3] Chinese Astronaut Res & Training Ctr, State Key Lab Space Med Fundamentals & Applicat, Beijing, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 153卷 / 01期

关键词：

Surface plasmon resonance; Self-assembled monolayer; 3-Nitrotyrosine; Urine; Immunosensor; LIQUID-CHROMATOGRAPHY; OXIDATIVE STRESS; ELECTROCHEMICAL DETECTION; IMMUNOSENSOR; NITROTYROSINE; SIMAZINE; ANTIBODY; TYROSINE; SENSORS; ASSAY;

D O I：

10.1016/j.snb.2010.10.022

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the detection of a low-molecular weight molecule, 3-nitrotyrosine (3-NT) (similar to 226 Da), in human urine by coupling indirect inhibition assay with a surface plasmon resonance (SPR) sensor. 3-NT antibody (anti-3-NT Ab, mouse IgG) was used in this assay. An optimal antibody concentration has been measured at 27.9 mu g/mL in order to obtain the best performance of the sensor surface. The lowest detection limit for 3-NT with this method is 4.7 ng/mL (S/N = 3). Sensor reliability was demonstrated by good specificity, intra-assay and inter-assay relative standard deviations <8%, average recovery of 107.68 +/- 19.4% and sensor surface (self-assembled monolayer) stability through more than 200 regeneration cycles and 15 clays of repeated measurement. This is the first SPR biosensor assay of 3-NT in human urine. The high stability of the SPR sensor surface underlies the potential of the SPR method as a low cost diagnostic tool for clinical detection of 3-NT. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：164 / 169

页数：6

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