The inhibitor protein of the F1F0-ATP synthase is associated to the external surface of endothelial cells

被引：30
作者：
Cortés-Hernandez, P
Domínguez-Ramírez, L
Estrada-Bernal, A
Montes-Sánchez, DG
Zentella-Dehesa, A
de Gómez-Puyou, MT
Gómez-Puyou, A
García, JJ [1 ]
机构：
[1] Inst Nacl Cardiol Ignacio Chavez, Mexico City, DF, Mexico
[2] Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City, DF, Mexico
[3] Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Mexico City, DF, Mexico
[4] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 330卷 / 03期
关键词：
ATP synthase; inhibitor protein; endothelial cells; TNF-alpha; plasma membrane;
D O I：
10.1016/j.bbrc.2005.03.064
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ATPase inhibitor protein (IP) of mitochondria was detected in the plasma membrane of living endothelial cells by flow cytometry, competition assays, and confocal microscopy of cells exposed to IP antibodies. The plasma membranes of endothelial cells also possess beta-subunits of the mitochondrial ATPase. Plasma membranes have the capacity to bind exogenous IP. TNF-alpha decreases the level of beta-subunits and increases the amount of IP, indicating that the ratio of IP to beta-subunit exhibits significant variations. Therefore, it is probable that the function of IP in the plasma membrane of endothelial cells is not limited to regulation of catalysis. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：844 / 849
页数：6
相关论文
共 27 条
[1]  
Arakaki N, 2003, MOL CANCER RES, V1, P931
[2]   BINDING OF DICYCLOHEXYLCARBODIIMIDE TO A NATIVE F1-ATPASE - INHIBITOR PROTEIN COMPLEX ISOLATED FROM BOVINE HEART-MITOCHONDRIA [J].
BELTRAN, C ;
GOMEZPUYOU, A ;
DEGOMEZPUYOU, MT .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 152 (02) :867-873
[3]   An inhibitor of the F1 subunit of ATP synthase (IF1) modulates the activity of angiostatin on the endothelial cell surface [J].
Burwick, NR ;
Wahl, ML ;
Fang, J ;
Zhong, Z ;
Moser, TL ;
Li, B ;
Capaldi, RA ;
Kenan, DJ ;
Pizzo, SV .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (03) :1740-1745
[4]   Modulation of the oligomerization state of the bovine F1-ATPase inhibitor protein, IF1, by pH [J].
Cabezon, E ;
Butler, PJG ;
Runswick, MJ ;
Walker, JE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (33) :25460-25464
[5]   The structure of bovine F1-ATPase in complex with its regulatory protein IF1 [J].
Cabezón, E ;
Montgomery, MG ;
Leslie, AGW ;
Walker, JE .
NATURE STRUCTURAL BIOLOGY, 2003, 10 (09) :744-750
[6]   The structure of bovine IF1, the regulatory subunit of mitochondrial F-ATPase [J].
Cabezón, E ;
Runswick, MJ ;
Leslie, AGW ;
Walker, JE .
EMBO JOURNAL, 2001, 20 (24) :6990-6996
[7]   A NOVEL LIGAND IN LYMPHOCYTE-MEDIATED CYTOTOXICITY - EXPRESSION OF THE BETA-SUBUNIT OF H+ TRANSPORTING ATP SYNTHASE ON THE SURFACE OF TUMOR-CELL LINES [J].
DAS, B ;
MONDRAGON, MOH ;
SADEGHIAN, M ;
HATCHER, VB ;
NORIN, AJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 180 (01) :273-281
[8]  
DEGOMEZPUYOU MT, 1977, ARCH BIOCHEM BIOPHYS, V182, P82
[9]   Equilibrium between monomeric and dimeric mitochondrial F1-inhibitor protein complexes [J].
Domínguez-Ramírez, L ;
Mendoza-Hernandez, G ;
Carabez-Trejo, A ;
Gómez-Puyou, A ;
de Gómez-Puyou, MT .
FEBS LETTERS, 2001, 507 (02) :191-194
[10]   NF-κB dependent activation of human endothelial cells treated with soluble products derived from human lymphomas [J].
Estrada-Bernal, A ;
Mendoza-Milla, C ;
Ventura-Gallegos, JL ;
López-Bojórquez, LN ;
Miranda-Peralta, E ;
Arechavaleta-Velasco, F ;
Vadillo-Ortega, F ;
Sánchez-Sánchez, L ;
Zentella-Dehesa, A .
CANCER LETTERS, 2003, 191 (02) :239-248
123