16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus

被引：38

作者：

Daffonchio, D ^{[1
]}

Borin, S ^{[1
]}

Consolandi, A ^{[1
]}

Mora, D ^{[1
]}

Manachini, PL ^{[1
]}

Sorlini, C ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Sci & Tecnol Alimentari & Microbiol, I-20133 Milan, Italy

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 163卷 / 02期

关键词：

Bacillus; 16S rRNA group I; identification; internal transcribed spacer polymerase chain reaction fingerprinting; single-strand conformation polymorphism; restriction analysis;

D O I：

10.1016/S0378-1097(98)00168-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The internal transcribed spacers between the 16S and the 23S ribosomal RNA genes were used to discriminate species of the 16S rRNA group I of the genus Bacillus by PCR. The spacer-PCR fingerprints clearly discriminated the different species, except those closely related like the members of the 'B. cereus group' (B. cereus, B. thuringiensis and B. mycoides) and the species of the 'B. subtilis group' (B. amyloliquefaciens and B. licheniformis). Examining in more detail the shortest internal transcribed spacers, B. subtilis group species were distinguished by single-strand conformation polymorphism analysis, whereas B. mycoides was differentiated from B. cereus/B. thuringiensis by restriction analysis. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：229 / 236

页数：8

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