Effects of starvation on oxygen consumption, ammonia excretion and biochemical composition of the hepatopancreas on adult males of the False Southern King crab Paralomis granulosa (Crustacea, Decapoda)

Adults of the False Southern King crab, Paralomis granulosa, were starved between 0 and 12 days to evaluate the impact of fasting on the oxygen consumption, nitrogen excretion, O/N ratio and changes on biochemical composition of the hepatopancreas. During the experiment, no mortalities were recorded;, physiological changes were detected after 6 days of fasting with an increase of nitrogen excretion (pless than or equal to0.05). After 9 days of starvation, the crabs showed a maximum decrease in the lipid content (4.3 +/- 1.2%, p<0.05), accompanied by an increase in oxygen consumption (53.1 +/- 10.9 Ag O-2 h(-1) g(-1)). The lowest O/N ratio was detected after 6 days (6.4 +/- 4.8) and the highest after 12 days of fasting (38.1 +/- 20.4), indicating that initially crabs utilized proteins as source of energy, followed by lipids. Moreover, after 12 days, there was a significant increase in the hepatosomatic index (HI) and total lipid content (9.7 +/- 1.0%,p<0.05), which could be associated with the reabsorption of other tissues to the hepatopancreas. Our results provide new information on this species that shows a different pattern of adaptation for each period of starvation and a good correlation between physiological and biochemical parameters. The ability to withstand and recover from periods of nutritional stress is an important adaptation for survival of any organism that must sporadically endure periods of limited food supply. (C) 2004 Elsevier Inc. All rights reserved.