Transcriptome-wide miRNA identification of Bacopa monnieri: a cross-kingdom approach

被引：23

作者：

Gadhavi, Harshida ^{[1
]}

Patel, Maulikkumar ^{[1
]}

Mangukia, Naman ^{[1
]}

Shah, Kanisha ^{[2
]}

Bhadresha, Kinjal ^{[2
]}

Patel, Saumya K. ^{[1
]}

Rawal, Rakesh M. ^{[2
]}

Pandya, Himanshu A. ^{[1
]}

机构：

[1] Gujarat Univ, Sch Sci, Dept Bot Bioinformat & Climate Change Impacts Man, Ahmadabad, Gujarat, India

[2] Gujarat Univ, Sch Sci, Dept Life Sci Food Sci & Nutr, Ahmadabad, Gujarat, India

来源：

PLANT SIGNALING & BEHAVIOR | 2020年 / 15卷 / 01期

关键词：

Bacopa monnieri; miRNA; Cross-kingdom; Transcriptome; Medicinal Plant; MICRORNA; TARGETS;

D O I：

10.1080/15592324.2019.1699265

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacopa monnieri known as 'Brahmi' is a well-known medicinal plant belonging to Scrophulariaceae family for its nootropic properties. To the best of our knowledge, no characterization data is available on the potential role of micro RNAs (miRNAs) from this plant till date. We present here the first report of computational characterizations of miRNAs from B. monnieri. Owing to the high conservation of miRNAs in nature, new and potential miRNAs can be identified in plants using in silico techniques. Using the plant miRNA sequences present in the miRBase repository, a total of 12 miRNAs were identified from B. monnieri which pertained to 11 miRNA families from the shoot and root transcriptome data. Furthermore, gene ontology analysis of the identi?ed 68 human target genes exhibited significance in various biological processes. These human target genes were associated with signaling pathways like NF-kB and MAPK with TRAF2, CBX1, IL1B, ITGA4 and ITGB1BP1 as the top five hub nodes. This cross-kingdom study provides initial insights about the potential of miRNA-mediated cross-kingdom regulation and unravels the essential target genes of human with implications in numerous human diseases including cancer.

引用

页数：16

共 45 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].