Molecular characterization of Thai Ehrlichia canis and Anaplasma platys strains detected in dogs

被引：54

作者：

Pinyoowong, Danai ^{[1
]}

Jittapalapong, Sathaporn ^{[2
]}

Suksawat, Fanan ^{[3
]}

Stich, Roger W. ^{[4
]}

Thamchaipenet, Arinthip ^{[1
]}

机构：

[1] Kasetsart Univ, Fac Sci, Dept Genet, Bangkok, Thailand

[2] Kasetsart Univ, Fac Vet Med, Dept Parasitol, Bangkok, Thailand

[3] Khon Kaen Univ, Fac Vet Med, Dept Med, Khon Kaen, Thailand

[4] Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA

来源：

INFECTION GENETICS AND EVOLUTION | 2008年 / 8卷 / 04期

关键词：

Ehrlichia canis; Anaplasma platys; 16S rDNA; phylogenetic tree; dogs; Bangkok; Thailand;

D O I：

10.1016/j.meegid.2007.06.002

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Canine monocytic ehrlichiosis caused by Ehrlichia canis is of veterinary importance worldwide. In Thailand, there has been little information available on E. canis and its phylogeny. The objective of this study was to characterize and establish molecular structure and phylogeny of Thai Ehrlichia and Anaplasma strains. Genus-specific primers for Ehrlichia and Anaplasma were used to amplify the 16S rRNA gene from naturally infected canine blood samples, and these amplicon sequences were compared with other sequences from GenBank. Both homology and secondary structure analysis of 16S rRNA sequences indicated that they were novel E. canis and A. platys strains. Phylogenetic analysis revealed that the Thai E. canis strain was closely related and formed a single cluster with E. canis from different countries. A. platys found in this study showed close relationship with earlier report of A. platys from Thailand. To our knowledge this report represents the first molecular characterization of the nearly complete 16S rRNA gene from E. canis in dogs from Thailand. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：433 / 438

页数：6

共 23 条

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