Investigating the effect of polarity of stationary and mobile phases on retention of cannabinoids in normal phase liquid chromatography

被引：12

作者：

De Luca, Chiara ^{[1
]}

Buratti, Alessandro ^{[1
]}

Krauke, Yannick ^{[2
]}

Stephan, Svea ^{[2
]}

Monks, Kate ^{[2
]}

Brighenti, Virginia ^{[3
]}

Pellati, Federica ^{[3
]}

Cavazzini, Alberto ^{[1
]}

Catani, Martina ^{[1
]}

Felletti, Simona ^{[1
,2
]}

机构：

[1] Univ Ferrara, Dept Chem Pharmaceut & Agr Sci, Via L Borsari 46, I-44121 Ferrara, Italy

[2] KNAUER Wissensch Gerate GmbH, Hegauer Weg 38, D-14163 Berlin, Germany

[3] Univ Modena & Reggio Emilia, Dept Life Sci, Via G Campi 103, I-41125 Modena, Italy

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2022年 / 414卷 / 18期

关键词：

Cannabinoids; Cannabis sativa L; HPLC; Hemp; Normal phase; Polar-bonded phases; STRUCTURAL FEATURES; MODEL COMPOUNDS; HPLC METHOD; SELECTIVITY; SOLVENT; CYANO; AMINO; DIOL; SEPARATION; COLUMNS;

D O I：

10.1007/s00216-021-03862-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This work reports about a screening of four adsorbents with different polarity employed for the separation of the main phytocannabinoids contained in Cannabis sativa L., under normal phase liquid chromatography (NPLC). The effect of polarity and type of interaction mechanisms of the adsorbents (namely Si-, CN-, Diol-, and NH2-based SPs) on retention has been investigated under a variety of conditions either by using different combinations of apolar solvents (heptane or hexane) and alcohols (ethanol or isopropanol). The columns have also been employed for the separation of a real cannabis sample.

引用

页码：5385 / 5395

页数：11

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