Involvement of monocarboxylate transporter 1 (SLC16A1) in the uptake of L-lactate in human astrocytes

被引：10

作者：

Ideno, Masaya ^{[1
]}

Kobayashi, Masaki ^{[2
]}

Sasaki, Shotaro ^{[1
,3
]}

Futagi, Yuya ^{[1
]}

Narumi, Katsuya ^{[1
]}

Furugen, Ayako ^{[1
]}

Iseki, Ken ^{[1
,2
]}

机构：

[1] Hokkaido Univ, Fac Pharmaceut Sci, Div Pharmasci, Lab Clin Pharmaceut & Therapeut,Kita Ku, Kita 12 Jo,Nishi 6 Chome, Sapporo, Hokkaido 0600812, Japan

[2] Hokkaido Univ Hosp, Dept Pharm, Kita Ku, Ktia 14 Jo,Nishi 5 Chome, Sapporo, Hokkaido 0608648, Japan

[3] Toho Univ, Fac Pharmaceut Sci, Funabashi, Chiba 2748510, Japan

来源：

LIFE SCIENCES | 2018年 / 192卷

关键词：

Human astrocyte; Monocarboxylate transporter; L-lactate; FAMILY;

D O I：

10.1016/j.lfs.2017.10.022

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Purpose: Astrocytes, the most abundant glial cells in the central nervous system (CNS), help neurons survive. Monocarboxylate transporters (MCTs) are reported to transport L-lactate, which is important for CNS physiology and cognitive function. However, it remains unclear which MCT isoform is functionally expressed by human astrocytes. The aim of this study was to establish the contribution of each MCT isoform to L-lactate transport in human astrocytes. Methods: The function of L-lactate transport was studied using NHA cells as a human astrocyte model and radiolabeled L-lactate. The expression of MCT in human astrocytes was detected by immunohistochemistry staining. Results: The cellular uptake of L-lactate was found to be pH- and concentration-dependent with a Km value for L-lactate uptake of 0.64 mM. This Km was similar to what has been previously established for MCT1-mediated L-lactate uptake. alpha-Cyano-4-hydroxycinnamate (CHC) and 5-oxoproline, which are both MCT1 inhibitors, were found to significantly inhibit the uptake of L-lactate, suggesting MCT1 is primarily responsible for L-lactate transport. Moreover, MCT1 protein was expressed in human astrocytes. Conclusion: pH-dependent L-lactate transport is mediated by MCT1 in human astrocytes.

引用

页码：110 / 114

页数：5

共 19 条

[1] Early Decline in Glucose Transport and Metabolism Precedes Shift to Ketogenic System in Female Aging and Alzheimer's Mouse Brain: Implication for Bioenergetic Intervention [J].