Multicenter Evaluation of a New DNA Microarray for Rapid Detection of Clinically Relevant bla Genes from β-Lactam-Resistant Gram-Negative Bacteria

被引：33

作者：

Bogaerts, Pierre ^{[1
]}

Hujer, Andrea M. ^{[2
,3
]}

Naas, Thierry ^{[4
]}

de Castro, Roberta Rezende ^{[1
]}

Endimiani, Andrea ^{[2
,3
]}

Nordmann, Patrice ^{[4
]}

Glupczynski, Youri ^{[1
]}

Bonomo, Robert A. ^{[2
,3
]}

机构：

[1] Clin Univ UCL Mt Godinne, Bacteriol Lab, B-5530 Yvoir, Belgium

[2] Case Western Reserve Univ, Sch Med, Dept Med, Cleveland, OH 44106 USA

[3] Vet Affairs Med Ctr, Louis Stokes Cleveland Dept, Cleveland, OH USA

[4] Hop Bicetre, AP HP, Serv Bacteriol Virol,Fac Med Paris, INSERM Emerging Resistance Antibiot U914, F-94275 Le Kremlin Bicetre, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 09期

基金：

美国国家卫生研究院;

关键词：

ESCHERICHIA-COLI; CTX-M; BELGIAN HOSPITALS; CARBAPENEMASES; INFECTIONS; EMERGENCE; SHV; TEM;

D O I：

10.1128/AAC.00353-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new commercial low-density microarray which identifies common extended-spectrum beta-lactamase plasmid-mediated cephalosporinase genes, as well as carbapenemase (bla(KPC) and bla(NDM)) genes, was evaluated. We tested 207 clinical and reference/collection isolates of the Enterobacteriaceae possessing different bla genes. Overall, the sensitivity and specificity of the microarray were 100% for the detection of the plasmid-mediated bla(AmpC), bla(KPC), and bla(NDM) genes using bla gene sequencing as the reference method.

引用

页码：4457 / 4460

页数：4

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