Evaluation of Basophil Activation in Mastocytosis with Hymenoptera Venom Anaphylaxis

被引：26
作者：
Gonzalez-de-Olano, D. [2 ,3 ]
Alvarez-Twose, I. [1 ,3 ]
Morgado, J. M. [1 ,3 ]
Esteban Lopez, M. I. [4 ]
Vega Castro, A. [5 ]
Alonso Diaz de Durana, M. D. [6 ]
Sanchez-Munoz, L. [1 ,3 ]
Matito, A. [1 ,3 ]
de la Hoz Caballer, B. [3 ,7 ]
Sanz, M. L. [8 ]
Orfao, A. [3 ,9 ,10 ]
Escribano, L. [1 ,3 ]
机构：
[1] Hosp Virgen del Valle, Inst Estudios Mastocitosis Castilla La Mancha, Toledo, Spain
[2] Hosp Fuenlabrada, Allergy Unit, Madrid, Spain
[3] REMA, Madrid, Spain
[4] Hosp Gen Segovia, Dept Allergy, Segovia, Spain
[5] Hosp Guadalajara, Dept Allergy, Guadalajara, Spain
[6] Fdn Hosp Alcorcon, Allergy Unit, Madrid, Spain
[7] Hosp Ramon & Cajal, Dept Allergy, E-28034 Madrid, Spain
[8] Univ Navarra Clin, Allergy & Immunol Dept, Pamplona, Spain
[9] Univ Salamanca, Ctr Invest Canc IBMCC, Dept Med, E-37008 Salamanca, Spain
[10] Univ Salamanca, Serv Gen Citometria, E-37008 Salamanca, Spain
来源：
CYTOMETRY PART B-CLINICAL CYTOMETRY | 2011年 / 80B卷 / 03期
关键词：
anaphylaxis; basophil activation test; hymenoptera; immunotherapy; mastocytosis; MAST-CELLS; SPANISH NETWORK; CD63; EXPRESSION; IMMUNOPHENOTYPIC CHARACTERIZATION; IMMUNOGLOBULIN-E; ALLERGY; DIAGNOSIS; TESTS; POTENTIALS; MUTATION;
D O I：
10.1002/cyto.b.20577
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: Basophil activation tests (BATs) have been demonstrated to be useful in detecting IgE-mediated sensitization by measuring basophil activation surface markers (CD63 and CD203c). Hymenoptera venom is one of the best known mediators-release trigger in patients with systemic mastocytosis (SM). The aim of this study was to investigate the use of BATs as an additional diagnostic tool in patients with mastocytosis suffering from hymenoptera venom anaphylaxis (HVA). Methods: A total of 22 patients with history of HVA and SM, together with a group of 11 patients with HVA in whom SM was ruled out after a complete bone marrow study, were analyzed. Results: Among 11 SM patients who had specific serum IgE (sIgE) against hymenoptera venom and an evaluable BAT, a positive BAT was found in nine. Additionally, a positive BAT was detected in three of seven patients who had no sIgE. These three patients had low levels of total IgE compared with control population (mean of 20 vs. 78 IU/mL); one had discontinued immunotherapy after 5 years, when sIgE levels had turned negative, and, in the other two patients, BAT identified the culprit insect. Conclusions: BAT is a useful complementary diagnostic tool to sIgE in mastocytosis patients with HVA, and it may contribute to predict or confirm these nearly fatal reactions, especially before discontinuing venom immunotherapy in patients who are negative for skin tests or sIgE or display low total IgE levels; in such cases, it also provides evidence on the culprit insect prompting HVA. (C) 2010 International Clinical Cytometry Society
引用
收藏
页码：167 / 175
页数：9
相关论文
共 40 条
[1]   Prevalence of allergy and anaphylactic symptoms in 210 adult and pediatric patients with mastocytosis in Spain:: a study of the Spanish network on mastocytosis (REMA) [J].
de Olano, D. Gonzalez ;
Caballer, B. de la Hoz ;
Lopezz, R. Nunez ;
Munozz, L. Sanchez ;
Agustin, M. Cuevas ;
Dieguez, M. C. ;
Twose, I. Alvarez ;
Castells, M. C. ;
Mora, L. Escribano .
CLINICAL AND EXPERIMENTAL ALLERGY, 2007, 37 (10) :1547-1555
[2]   Safety and effectiveness of immunotherapy in patients with indolent systemic mastocytosis presenting with Hymenoptera venom anaphylaxis [J].
De Olano, David Gonzalez ;
Alvarez-Twose, Ivan ;
Esteban-Lopez, Maria I. ;
Sanchez-Munoz, Laura ;
Durana, Maria D. Alonso Diaz ;
Vega, Arantza ;
Garcia-Montero, Andres ;
Gonzalez-Mancebo, Eloina ;
Belver, Teresa ;
Herrero-Gil, Maria D. ;
Fernandez-Rivas, Montserrat ;
Orfao, Alberto ;
de la Hoz, Belen ;
Castells, Mariana C. ;
Escribano, Luis .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2008, 121 (02) :519-526
[3]   Diagnostic tests based on human basophils: More potentials and perspectives than pitfalls [J].
de Weck, A. L. ;
Sanz, M. L. ;
Gamboa, P. M. ;
Aberer, W. ;
Bienvenu, J. ;
Blanca, M. ;
Demoly, P. ;
Ebo, D. G. ;
Mayorga, L. ;
Monneret, G. ;
Sainte-Laudy, J. .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2008, 146 (03) :177-189
[4]   Basophil-activation tests in hymenoptera allergy [J].
Dubois, Anthony E. J. ;
van der Heide, Sicco .
CURRENT OPINION IN ALLERGY AND CLINICAL IMMUNOLOGY, 2007, 7 (04) :346-349
[5]  
Eberlein-König B, 2006, J INVEST ALLERG CLIN, V16, P5
[6]  
Eberlein-König B, 2004, J INVEST ALLERG CLIN, V14, P10
[7]   Flow-assisted quantification of in vitro activated basophils in the diagnosis of wasp venom allergy and follow-up of wasp venom immunotherapy [J].
Ebo, D. G. ;
Hagendorens, M. M. ;
Schuerwegh, A. J. ;
Beirens, L. M. -N. ;
Bridts, C. H. ;
De Clerck, L. S. ;
Stevens, W. J. .
CYTOMETRY PART B-CLINICAL CYTOMETRY, 2007, 72B (03) :196-203
[8]   In vitro allergy diagnosis:: should we follow the flow? [J].
Ebo, DG ;
Hagendorens, MM ;
Bridts, CH ;
Schuerwegh, AJ ;
De Clerck, LS ;
Stevens, WJ .
CLINICAL AND EXPERIMENTAL ALLERGY, 2004, 34 (03) :332-339
[9]   The basophil activation test in wasp venom allergy: sensitivity, specificity and monitoring specific immunotherapy [J].
Erdmann, SM ;
Sachs, B ;
Kwiecien, R ;
Moll-Slodowy, S ;
Sauer, I ;
Merk, HF .
ALLERGY, 2004, 59 (10) :1102-1109
[10]   Indolent systemic mast cell disease in adults:: Immunophenotypic characterization of bone marrow mast cells and its diagnostic implications [J].
Escribano, L ;
Orfao, A ;
Díaz-Agustin, B ;
Villarrubia, J ;
Cerveró, C ;
López, A ;
Marcos, MAG ;
Bellas, C ;
Fernández-Cañadas, S ;
Cuevas, M ;
Sánchez, A ;
Velasco, JL ;
Navarro, JL ;
San Miguel, JF .
BLOOD, 1998, 91 (08) :2731-2736
1234