Incorporation of Lysyl-tRNA synthetase into human immunodeficiency virus type 1

被引:107
作者
Cen, S
Khorchid, A
Javanbakht, H
Gabor, J
Stello, T
Shiba, K
Musier-Forsyth, K
Kleiman, L
机构
[1] Sir Mortimer B Davis Jewish Hosp, Lady Davis Inst Med Res, Montreal, PQ H3T 1E2, Canada
[2] Sir Mortimer B Davis Jewish Hosp, McGill AIDS Ctr, Montreal, PQ H3T 1E2, Canada
[3] McGill Univ, Dept Med, Montreal, PQ H3T 1E2, Canada
[4] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3T 1E2, Canada
[5] Japanese Fdn Canc Res, Inst Canc, Dept Cell Biol, Toshima Ku, Tokyo 170, Japan
[6] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
关键词
D O I
10.1128/JVI.75.11.5043-5048.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
During human immunodeficiency virus type 1 (HIV-1) assembly, tRNA(Lys) isoacceptors are selectively incorporated into virions and tRNA(3)(Lys) is used as the primer for reverse transcription. We show herein that the tRNA(Lys)-binding protein, lysyl-tRNA synthetase (LysRS), is also selectively packaged into HIV-1. The viral precursor protein Pr55(gag) alone will package LysRS into Pr55(gag) particles, independently of tRNA(Lys). With the additional presence of the viral precursor protein Pr160(gag-pol), tRNA(Lys) and LysRS are both packaged into the particle. While the predominant cytoplasmic LysRS has an apparent M-r of 70,000, viral LysRS associated with tRNA(Lys) packaging is shorter, with an apparent M-r of 63,000. The truncation occurs independently of viral protease and might be required to facilitate interactions involved in the selective packaging and genomic placement of primer tRNA(3)(Lys).
引用
收藏
页码:5043 / 5048
页数:6
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