A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain

被引：34

作者：

Rust, Ruslan ^{[1
,2
,3
,4
]}

Kirabali, Tunahan ^{[1
,3
,4
]}

Groennert, Lisa ^{[1
]}

Dogancay, Berre ^{[3
,4
]}

Limasale, Yanuar D. P. ^{[5
]}

Meinhardt, Andrea ^{[5
]}

Werner, Carsten ^{[5
]}

Lavina, Barbara ^{[6
]}

Kulic, Luka ^{[1
,3
,4
]}

Nitsch, Roger M. ^{[1
,3
,4
]}

Tackenberg, Christian ^{[1
,3
,4
]}

Schwab, Martin E. ^{[1
,2
,3
,4
]}

机构：

[1] Univ Zurich, Inst Regenerat Med, Zurich, Switzerland

[2] Swiss Fed Inst Technol, Dept Hlth Sci & Technol, Zurich, Switzerland

[3] Univ Zurich, Neurosci Ctr Zurich, Zurich, Switzerland

[4] Swiss Fed Inst Technol, Zurich, Switzerland

[5] Leibniz Inst Polymer Res Dresden, Dresden, Germany

[6] Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden

来源：

FRONTIERS IN NEUROSCIENCE | 2020年 / 14卷

关键词：

quantification; image processing; angiogenesis; blood vessels; stroke; development; central nervous system (CNS); WHITE-MATTER; NEUROVASCULAR UNIT; CEREBRAL-CORTEX; DYSFUNCTION; BARRIER; DISEASE; NEURODEGENERATION; QUANTIFICATION; ANGIOGENESIS; MICROVESSELS;

D O I：

10.3389/fnins.2020.00244

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The distinct organization of the brain's vasculature ensures the adequate delivery of oxygen and nutrients during development and adulthood. Acute and chronic pathological changes of the vascular system have been implicated in many neurological disorders including stroke and dementia. Here, we describe a fast, automated method that allows the highly reproducible, quantitative assessment of distinct vascular parameters and their changes based on the open source software Fiji (ImageJ). In particular, we developed a practical guide to reliably measure aspects of growth, repair and maturation of the brain's vasculature during development and neurovascular disease in mice and humans. The script can be used to assess the effects of different external factors including pharmacological treatments or disease states. Moreover, the procedure is expandable to blood vessels of other organs and vascular in vitro models.

引用

页数：10

共 53 条

[1] Redefining malignant pleural mesothelioma types as a continuum uncovers immune-vascular interactions [J].

Alcala, Nicolas ;

Mangiante, Lise ;

Le-Stang, Nolwenn ;

Gustafson, Corinne E. ;

Boyault, Sandrine ;

Damiola, Francesca ;

Alcala, Karine ;

Brevet, Marie ;

Thivolet-Bejui, Francoise ;

Blanc-Fournier, Cecile ;

Le Rochais, Jean-Philippe ;

Planchard, Gaetane ;

Rousseau, Nathalie ;

Damotte, Diane ;

Pairon, Jean Claude ;

Copin, Marie Christine ;

Scherpereel, Arnaud ;

Wasielewski, Eric ;

Wicquart, Laurence ;

Lacomme, Stephanie ;

Vignaud, Jean-Michel ;

Ancelin, Gaspard ;

Girard, Cecile ;

Sagan, Christine ;

Bonnetaud, Christelle ;

Hofman, Veronique ;

Hofman, Paul ;

Mouroux, Jerome ;

de Montpreville, Vincent Thomas ;

Clermont-Taranchon, Estelle ;

Mazieres, Julien ;

Rouquette, Isabelle ;

Begueret, Hugues ;

Blay, Jean-Yves ;

Lantuejoul, Sylvie ;

Bueno, Raphael ;

Caux, Christophe ;

Girard, Nicolas ;

McKay, James D. ;

Foll, Matthieu ;

Galateau-Salle, Francoise ;

Fernandez-Cuesta, Lynnette .

EBIOMEDICINE, 2019, 48 :191-202

[2] STAGING OF ALZHEIMERS-DISEASE-RELATED NEUROFIBRILLARY CHANGES [J].

BRAAK, H ;

BRAAK, E .

NEUROBIOLOGY OF AGING, 1995, 16 (03) :271-278

[3] Pericytes and Neurovascular Function in the Healthy and Diseased Brain [J].

Brown, Lachlan S. ;

Foster, Catherine G. ;

Courtney, Jo-Maree ;

King, Natalie E. ;

Howells, David W. ;

Sutherland, Brad A. .

FRONTIERS IN CELLULAR NEUROSCIENCE, 2019, 13

[4] The matricellular protein CCN1 controls retinal angiogenesis by targeting VEGF, Src homology 2 domain phosphatase-1 and Notch signaling [J].

Chintala, Hemabindu ;

Krupska, Izabela ;

Yan, Lulu ;

Lau, Lester ;

Grant, Maria ;

Chaqour, Brahim .

DEVELOPMENT, 2015, 142 (13) :2364-+

[5] 3D surface analysis of hippocampal microvasculature in the irradiated brain [J].

Craver, Brianna M. ;

Acharya, Munjal M. ;

Allen, Barrett D. ;

Benke, Sarah N. ;

Hultgren, Nan W. ;

Baulch, Janet E. ;

Limoli, Charles L. .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2016, 57 (05) :341-349

[6] Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO [J].

Ertuerk, Ali ;

Lafkas, Daniel ;

Chalouni, Cecile .

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (89)

[7] Roles of Pericytes in Stroke Pathogenesis [J].

Gautam, Jyoti ;

Yao, Yao .

CELL TRANSPLANTATION, 2018, 27 (12) :1798-1808

[8] Accelerated pericyte degeneration and blood-brain barrier breakdown in apolipoprotein E4 carriers with Alzheimer's disease [J].

Halliday, Matthew R. ;

Rege, Sanket V. ;

Ma, Qingyi ;

Zhao, Zhen ;

Miller, Carol A. ;

Winkler, Ethan A. ;

Zlokovic, Berislav V. .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2016, 36 (01) :216-227

[9] White matter capillaries in vascular and neurodegenerative dementias [J].

Hase, Yoshiki ;

Ding, Ren ;

Harrison, Gina ;

Hawthorne, Emily ;

King, Amilia ;

Gettings, Sean ;

Platten, Charlotte ;

Stevenson, William ;

Craggs, Lucinda J. L. ;

Kalaria, Raj N. .

ACTA NEUROPATHOLOGICA COMMUNICATIONS, 2019, 7 :16

[10] National Institute on Aging-Alzheimer's Association guidelines for the neuropathologic assessment of Alzheimer's disease [J].

Hyman, Bradley T. ;

Phelps, Creighton H. ;

Beach, Thomas G. ;

Bigio, Eileen H. ;

Cairns, Nigel J. ;

Carrillo, Maria C. ;

Dickson, Dennis W. ;

Duyckaerts, Charles ;

Frosch, Matthew P. ;

Masliah, Eliezer ;

Mirra, Suzanne S. ;

Nelson, Peter T. ;

Schneider, Julie A. ;

Thal, Dietmar Rudolf ;

Thies, Bill ;

Trojanowski, John Q. ;

Vinters, Harry V. ;

Montine, Thomas J. .

ALZHEIMERS & DEMENTIA, 2012, 8 (01) :1-13

← 1 2 3 4 5 6 →