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Effect of Cationic Surfactants on the Enzymatic Activity of α-Chymotrypsin
被引:11
|作者:
Verma, S. K.
[1
]
Ghosh, K. K.
[1
]
机构:
[1] Pt Ravishankar Shukla Univ, Sch Studies Chem, Raipur 492010, CG, India
关键词:
INTERFACIAL BINDING;
AQUEOUS-SOLUTIONS;
HYDROLYSIS;
KINETICS;
ACETATE;
MECHANISM;
MICELLES;
DYNAMICS;
LIPASE;
D O I:
10.1134/S0023158411010216
中图分类号:
O64 [物理化学(理论化学)、化学物理学];
学科分类号:
070304 ;
081704 ;
摘要:
The hydrolysis of p-nitrophenyl benzoate catalyzed by alpha-chymotrypsin in the presence of cetyltriphenylphosphonium bromide, cetyltributylphosphonium bromide and cetyltrimethylammonium bromide (pre and post micellar regions) has been studied. The ester is hydrolyzed readily by alpha-chymotrypsin in all the surfactants with the highest activity shown in cetyltributylphosphonium bromide. The dependences of the Michaelis constant and the catalytic constant with surfactant concentration have also been discussed.
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页码:6 / 10
页数:5
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