The hydrolysis of p-nitrophenyl benzoate catalyzed by alpha-chymotrypsin in the presence of cetyltriphenylphosphonium bromide, cetyltributylphosphonium bromide and cetyltrimethylammonium bromide (pre and post micellar regions) has been studied. The ester is hydrolyzed readily by alpha-chymotrypsin in all the surfactants with the highest activity shown in cetyltributylphosphonium bromide. The dependences of the Michaelis constant and the catalytic constant with surfactant concentration have also been discussed.
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Xian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
Petr Univ China Huadong, Sch Petr Engn, Qingdao 266580, Peoples R ChinaXian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
Bai, Yun
Zhang, Jie
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Xian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R ChinaXian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
Zhang, Jie
Dong, Sanbao
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Xian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R ChinaXian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
Dong, Sanbao
Li, Jinling
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Xian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
CNPC Res Inst Safety & Environm Technol, State Key Lab Petr Pollut Control, Beijing 102206, Peoples R ChinaXian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
Li, Jinling
Zhang, Rongjun
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机构:
Xian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
CNPC Res Inst Safety & Environm Technol, State Key Lab Petr Pollut Control, Beijing 102206, Peoples R ChinaXian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
Zhang, Rongjun
Pu, Chunsheng
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Petr Univ China Huadong, Sch Petr Engn, Qingdao 266580, Peoples R ChinaXian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
Pu, Chunsheng
Chen, Gang
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机构:
Xian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China
CNPC Res Inst Safety & Environm Technol, State Key Lab Petr Pollut Control, Beijing 102206, Peoples R ChinaXian Shiyou Univ, Shaanxi Prov Key Lab Environm Pollut Control & Re, Xian 710065, Peoples R China