Volume-sensitive chloride channels are involved in cisplatin treatment of osteosarcoma

被引:19
|
作者
Cai, Siyi [1 ,2 ]
Zhang, Tao [3 ]
Zhang, Dandan [4 ]
Qiu, Guixing [1 ,2 ]
Liu, Yong [1 ,2 ]
机构
[1] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Orthoped, Beijing 100032, Peoples R China
[2] Peking Union Med Coll, Beijing 100032, Peoples R China
[3] Jinan Univ, Dept Internal Med, Affiliated Hosp 1, Guangzhou 510630, Guangdong, Peoples R China
[4] Jinan Univ, Coll Med, Dept Histol & Embryol, Guangzhou 510632, Guangdong, Peoples R China
关键词
cisplatin; chloride channel; osteosarcoma; CELL-CYCLE; DIFFERENTIAL EXPRESSION; CL-CHANNEL; CLC-3; PROLIFERATION; DECREASE; CURRENTS; ACTIVATION; RESISTANCE; POTASSIUM;
D O I
10.3892/mmr.2014.3068
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Chemotherapy is the most common therapeutic strategy used to treat osteosarcoma. The present study aimed to investigate the effects of functionally activated chloride channels on cisplatin-induced apoptosis of MG-63 human osteosarcoma cells. An MTT assay and flow cytometry were used to detect proliferation and apoptosis of the cells, respectively. Live cell imaging was used to detect volume changes in response to treatment with cisplatin and/or chloride channel blockers. The effects of these treatments on chloride currents were also assayed using the patch-clamp technique. The results of the present study indicate that chloride channel blockers may suppress cisplatin-induced apoptosis. The MG-63 cells cultured with cisplatin demonstrated an apoptotic volume decrease, as well as suppression of cell proliferation; which were reversed by co-treatment with chloride channel blockers. These results suggest that cisplatin may activate chloride channels, and that channel activation is an early signal in the pathways that lead to cisplatin-induced apoptosis and inhibition of proliferation in MG-63 cells. In conclusion, these results indicate that chloride channels have an important role in cisplatin treatment of osteosarcoma.
引用
收藏
页码:2465 / 2470
页数:6
相关论文
共 50 条
  • [21] A VOLUME-SENSITIVE EPITHELIAL CHLORIDE CONDUCTANCE
    BUTT, AG
    WORRELL, RT
    FRIZZELL, RA
    FASEB JOURNAL, 1988, 2 (05): : A1284 - A1284
  • [22] Is autocrine ATP release required for activation of volume-sensitive chloride channels? Reply
    MacVicar, BA
    JOURNAL OF NEUROPHYSIOLOGY, 2003, 90 (04) : 2792 - 2793
  • [23] Volume-sensitive chloride channels in the primary culture cells of human cervical carcinoma
    Shen, MR
    Wu, SN
    Chou, CY
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1996, 1315 (02): : 138 - 144
  • [24] MDR 1 and volume-sensitive chloride channel
    Okada, Y
    NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 1997, 71 (08): : 789 - 792
  • [25] Volume-sensitive chloride channels do not mediate activation-induced chloride efflux in human neutrophils
    Perez-Cornejo, P
    Arreola, J
    Law, FY
    Schultz, JB
    Knauf, PA
    JOURNAL OF IMMUNOLOGY, 2004, 172 (11): : 6988 - 6993
  • [26] Impaired activity of volume-sensitive Cl- channel is involved in cisplatin resistance of cancer cells
    Lee, Elbert L.
    Shimizu, Takahiro
    Ise, Tomoko
    Numata, Tomohiro
    Kohno, Kimitoshi
    Okada, Yasunobu
    JOURNAL OF CELLULAR PHYSIOLOGY, 2007, 211 (02) : 513 - 521
  • [27] NORMOTONIC ACTIVATION OF VOLUME-SENSITIVE Cl- CHANNELS IS INVOLVED IN CELL VOLUME MAINTAINING AND DEVELOPMENT OF NASOPHARYNGEAL CARCINOMA
    Chen, Lixin
    Zhu, Linyan
    Mao, Jianwen
    Yang, Linjie
    Jiao, Chenggang
    Zuo, Wanhong
    He, Shutong
    Jacob, Tim J. C.
    Wang, Liwei
    JOURNAL OF PHYSIOLOGICAL SCIENCES, 2009, 59 : 392 - 392
  • [28] Propofol inhibits volume-sensitive chloride channels in human coronary artery smooth muscle cells
    Masuda, T
    Tomiyama, Y
    Kitahata, H
    Kuroda, Y
    Oshita, S
    ANESTHESIA AND ANALGESIA, 2003, 97 (03): : 657 - 662
  • [29] Cellular and molecular physiology of volume-sensitive anion channels
    Strange, K
    Emma, F
    Jackson, PS
    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1996, 270 (03): : C711 - C730
  • [30] Hypotonic activation of volume-sensitive outwardly rectifying chloride channels in cultured PASMCs is modulated by SGK
    Wang, GX
    McCrudden, C
    Dai, YP
    Horowitz, B
    Hume, JR
    Yamboliev, IA
    AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2004, 287 (02): : H533 - H544