Anti-Inflammatory and Antioxidant Properties of Dehydrated Potato-Derived Bioactive Compounds in Intestinal Cells

被引：20

作者：

Basilicata, Manuela Giovanna ^{[1
,2
]}

Pepe, Giacomo ^{[1
]}

Rapa, Shara Francesca ^{[1
]}

Merciai, Fabrizio ^{[1
,2
]}

Ostacolo, Carmine ^{[3
]}

Manfra, Michele ^{[4
]}

Di Sarno, Veronica ^{[1
]}

Autore, Giuseppina ^{[1
]}

De Vita, Daniela ^{[5
]}

Marzocco, Stefania ^{[1
]}

Campiglia, Pietro ^{[1
,6
]}

机构：

[1] Univ Salerno, Sch Pharm, Dept Pharm, Via Giovanni Paolo II 132, I-84084 Fisciano, Italy

[2] Univ Salerno, PhD Program Drug Discovery & Dev, Via Giovanni Paolo II 132, I-84084 Fisciano, Italy

[3] Univ Naples Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy

[4] Univ Basilicata, Dept Sci, Viale Ateneo Lucano 10, I-85100 Potenza, Italy

[5] Univ Roma La Sapienza, Dept Environm Biol, I-00185 Rome, Italy

[6] European Biomed Res Inst Salerno, Via De Renzi 50, I-84125 Salerno, Italy

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 23期

关键词：

bioactive peptides; in vitro gastrointestinal digestion; intestinal epithelial cells; inflammation; oxidative stress; potato proteins; INFLAMMATORY-BOWEL-DISEASE; OXIDATIVE STRESS; HEALTH; PRODUCTS; PROTEINS; INHIBITOR; PEPTIDES; BARRIER;

D O I：

10.3390/ijms20236087

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inflammation and oxidative stress are always more recognized as responsible for chronic disease at the intestinal level. Currently, a growing interest is addressed to the discovery of diet-derived products which have anti-inflammatory and antioxidant properties. This work aims to characterize the pharmacological potential of dehydrated potatoes. For this purpose, a simulated gastrointestinal digestion was carried out. The bioaccessible peptides were fractionated on the basis of their molecular weight and tested on intestinal epithelial cells (IEC-6) under oxidative and inflammatory conditions. Our results demonstrate that the tested peptide fractions were able to significantly inhibit tumor necrosis factor-alpha release and cycloxygenase-2 and inducible nitric oxide synthase expression. The tested peptides also showed significant antioxidant activity, being able to both reduce reactive oxygen species (ROS) release, also from mitochondria, and nitrotyrosine formation, and increase the antioxidant response by heme oxygenase-1 and superoxide dismutase expression. Moreover, the peptide fractions were able to significantly increase the wound repair in IEC-6. The obtained results indicate the anti-inflammatory and antioxidant potential of dehydrated potatoes at the intestinal level.

引用

页数：15

共 51 条

[1] Effect of Indoxyl Sulfate on the Repair and Intactness of Intestinal Epithelial Cells: Role of Reactive Oxygen Species' Release [J].