Evidence for a pool of coronin in mammalian cells that is sensitive to PI 3-kinase

被引：14

作者：

Didichenko, SA

Segal, AW

Thelen, M

机构：

[1] Inst Res Biomed, CH-6501 Bellinzona, Switzerland

[2] UCL, Dept Med, London WC1E 6JJ, England

来源：

FEBS LETTERS | 2000年 / 485卷 / 2-3期

关键词：

coronin; phosphatidylinositol; 3-kinase; phagocytosis; macrophage;

D O I：

10.1016/S0014-5793(00)02220-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coronin, a 57 kDa actin binding protein elutes with an apparent molecular mass of 400-600 kDa from gel filtration columns. This fraction is not unrelated to the reported 200 kDa complex where coronin is associated with phox proteins of the NADPH-oxidase. Phosphatidylinositol 3-kinase (PI 3-kinase) solubilizes coronin from the 400-600 kDa complex, thus constitutive active PI 3-kinase is sufficient to disrupt the complex, whereas wortmannin stabilizes it. Conversely, the phox protein associated pool of coronin is PI 3-kinase independent. During phagocytosis coronin is recruited together with PI 3-kinase to membranes of nascent and early phagosomes colocalizing with the actin cytoskeleton, confirming that coronin contributes to phagocytosis, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

收藏

页码：147 / 152

页数：6

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