2'-modified oligoribonucleotides containing 1,2-diol and aldehyde groups. Synthesis and properties

被引：3

作者：

Khomyakova, E. A. ^{[1
,2
]}

Zubin, E. M. ^{[1
,2
]}

Pavlova, L. V. ^{[3
]}

Kazanova, E. V. ^{[4
]}

Smirnov, I. P. ^{[5
]}

Pozmogova, G. E. ^{[5
]}

Muller, S. ^{[6
]}

Dolinnaya, N. G. ^{[1
,2
]}

Kubareva, E. A. ^{[1
,2
]}

Hartmann, R. K. ^{[3
]}

Oretskaya, T. S. ^{[1
,2
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia

[2] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow 119991, Russia

[3] Univ Marburg, D-35037 Marburg, Germany

[4] Sci Technol Informat Ctr, Moscow, Russia

[5] Res Inst Phys Chem Med, Moscow, Russia

[6] Ernst Moritz Arndt Univ Greifswald, D-17487 Greifswald, Germany

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2012年 / 38卷 / 05期

关键词：

modified oligoribonucleotides; cross-linking; RNA-protein interactions; bacterial ribonuclease P; RNASE-P RNA; ESCHERICHIA-COLI; RIBONUCLEASE-P; ELECTROPHILIC GROUPS; NUCLEIC-ACIDS; IN-VIVO; PROTEIN; OLIGONUCLEOTIDES; SITE; PRECURSOR;

D O I：

10.1134/S1068162012050068

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1,2-Diol-oligoribonucleotides were prepared using fully protected 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxoethyl]uridine 3'-phosphoramidite. Incorporation of the modified uridine residue into oligonucleotide chains was not shown to significantly affect the thermal stability of RNA-RNA and RNA-DNA duplexes. Periodate oxidation of the 1,2-diol group resulted in reactive 2'-aldehyde oligoribonucleotides. These oligonucleotides were studied for their application in the affinity modification of RNA recognizing proteins with an example of bacterial ribonuclease P.

引用

页码：488 / 499

页数：12

共 31 条

[1] RNA PRECURSOR MOLECULES AND RIBONUCLEASES IN ESCHERICHIA-COLI [J].