Energy transfer chemiluminescence determination of lomefloxacin

A photodegradation reaction took place when the lomefloxacin (LFLX) was irradiated by ultraviolet radiation, and the photoproduct can form a complex with Tb3+. The complex can sensitize the chemiluminescence(CL) reaction of Ce(IV)-SO32-, its emission intensity increased about five times than the non-irradiated system. Based on the CL reaction, a new photochemical energy transfer CL method for the determination of LFLX was developed. The relative CL intensity was linear with the concentration of LFLX in the range of 8.0 x 10(-10) - 2.0 x 10(-5) mol/L. The detection limit is 2.5 x 10(-11) mol/L. The relative standard deviation for the determination of 5.0 x 10(-8) mol/L LFLX was 2.8% (n=11). The method was successfully applied to the determination of LFLX in biological fluid. The mechanism of this system is proposed according to the results of experiment.