Cibacron blue immobilized poly(glycidyl-methacrylate) nanobeads for albumin removal in proteome studies

被引：10
作者：
Andac, Muge [1 ]
机构：
[1] Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2015年 / 43卷 / 02期
关键词：
albumin depletion; dye affinity; poly glycidyl methacrylate nanobeads; proteomics; HUMAN SERUM SAMPLES; DYE-AFFINITY BEADS; DISPERSION POLYMERIZATION; METHACRYLATE) BEADS; PLASMA-PROTEINS; MAGNETIC-FIELD; CHROMATOGRAPHY; PURIFICATION; DEPLETION; F3GA;
D O I：
10.3109/21691401.2013.852102
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A new approach to albumin removal for proteome studies involving human plasma samples was presented with dye affinity poly glycidyl methacrylate (PGMA) nanobeads in an average size of 45 nm. The specific surface area of PGMA nanobeads was calculated as 2616 m(2)/g. Cibacron Blue F3GA (CB) was immobilized onto PGMA nanobeads as dye ligand, and CB immobilized PGMA (CB-PGMA) nanobeads were characterized by a serial processing. Albumin depletion efficiency of CB-PGMA nanobeads was investigated in human plasma sample and confirmed by two-dimensional gel electrophoresis.
引用
收藏
页码：133 / 139
页数：7
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