Direct binding of ligandin to uridine 5'-diphosphate glucuronosyltransferase 1A1

被引：4

作者：

Akizawa, Emi ^{[1
]}

Koiwai, Kotaro ^{[1
]}

Hayano, Takahide ^{[1
]}

Maezawa, So ^{[1
]}

Matsushita, Toyofumi ^{[1
]}

Koiwai, Osamu ^{[1
]}

机构：

[1] Tokyo Univ Sci, Fac Sci & Technol, Dept Appl Biol Sci, Noda, Chiba 2788510, Japan

来源：

HEPATOLOGY RESEARCH | 2008年 / 38卷 / 04期

关键词：

bilirubin; endoplasmic reticulum; glutathione S-transferase; hepatocytes; ligandin; uridine 5'-diphosphate-glucuronosyltransferase;

D O I：

10.1111/j.1872-034X.2007.00285.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Aim: Bilirubin, a final degradation product of heme produced mainly in the spleen, is carried to the liver through its binding to albumin in the blood circulation. After its transport to hepatocytes, ligandin (glutathione S-transferase; GST) carries bilirubin to the endoplasmic reticulum (ER). uridine 5'-diphosphate-glucuronosyltransferase 1A1 (UGT1A1) glucuronidates bilirubin for solubilization in the ER. Methods: By GST pull-down and co-immunoprecipitation assays, GSTA2, a member of the alpha-class of GST, was observed to directly bind to UGT1A1 through the region present inside the ER. Results: GSTA2 was detected in the microsomal fraction together with the cytosolic fraction after hepatocyte fractionation. Conclusion: These results strongly suggest that bilirubin is directly delivered to UGT1A1 from ligandin for glucuronidation.

引用

页码：402 / 409

页数：8

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