Nonequilibrium microtubule fluctuations in a model cytoskeleton

被引：147

作者：

Brangwynne, Clifford P. ^{[1
]}

Koenderink, Gijsje H. ^{[1
]}

MacKintosh, Frederick C. ^{[2
]}

Weitz, David A. ^{[1
,3
]}

机构：

[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[2] Vrije Univ Amsterdam, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands

[3] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

来源：

PHYSICAL REVIEW LETTERS | 2008年 / 100卷 / 11期

关键词：

D O I：

10.1103/PhysRevLett.100.118104

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Biological activity gives rise to nonequilibrium fluctuations in the cytoplasm of cells; however, there are few methods to directly measure these fluctuations. Using a reconstituted actin cytoskeleton, we show that the bending dynamics of embedded microtubules can be used to probe local stress fluctuations. We add myosin motors that drive the network out of equilibrium, resulting in an increased amplitude and modified time dependence of microtubule bending fluctuations. We show that this behavior results from steplike forces on the order of 10 pN driven by collective motor dynamics.

引用

页数：4

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