Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells

被引：29

作者：

Fassina, Lorenzo ^{[1
]}

Saino, Enrica ^{[2
]}

Visai, Livia ^{[2
]}

Avanzini, Maria Antonietta ^{[3
]}

De Angelis, Maria Gabriella Cusella ^{[2
]}

Benazzo, Francesco ^{[4
]}

Van Vlierberghe, Sandra ^{[5
]}

Dubruel, Peter ^{[5
]}

Magenes, Giovanni ^{[1
]}

机构：

[1] Univ Pavia, CIT, Dipartimento Informat & Sistemist, Via Ferrata 1, I-27100 Pavia, Italy

[2] Univ Pavia, CIT, Dipartimento Biochim, I-27100 Pavia, Italy

[3] IRCCS San Matteo Pavia, Oncoematol Pediatricaa, Pavia, Italy

[4] Univ Pavia, IRCCS San Matteo, Dipartimento SMEC, I-27100 Pavia, Italy

[5] Univ Ghent, Biomat Res Grp, B-9000 Ghent, Belgium

来源：

2010 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) | 2010年

关键词：

IN-VITRO; CALCIFICATION; DEGRADATION; HYDROGELS; COLLAGEN; PROTEIN;

D O I：

10.1109/IEMBS.2010.5627475

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility; in addition, the in vitro modification of biomaterials with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where differentiated human bone marrow stromal cells built their extracellular matrix onto gelatin cryogel scaffolds. In comparison with control conditions without differentiation medium, the use of a differentiation medium increased, in vitro, the coating of gelatin cryogel with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The differentiation medium aimed at obtaining a better in vitro modification of gelatin cryogel in terms of cell colonization and coating with osteogenic signals, like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.

引用

页码：247 / 250

页数：4

共 21 条

[1] Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: Further insights in the search for a fetal calf serum substitute [J].