Optimization of fermentation conditions for the production of ethanol from sago starch using response surface methodology

被引：128
|
作者：
Ratnam, BVV [1 ]
Rao, MN [1 ]
Rao, MD [1 ]
Rao, SS [1 ]
Ayyanna, C [1 ]
机构：
[1] Andhra Univ, Ctr Biotechnol, Dept Chem Engn, Coll Engn, Visakhapatnam 530003, Andhra Pradesh, India
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2003年 / 19卷 / 05期
关键词：
central composite design; ethanol; glucoamylase; response surface methodology; sago starch; simultaneous saccharification and fermentation (SSF); Zymomonas mobilis;
D O I：
10.1023/A:1025174731814
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The quantitative effects of temperature, pH and time of fermentation were investigated on simultaneous saccharification and fermentation (SSF) of ethanol from sago starch with glucoamylase (AMG) and Zymomonas mobilis ZM4 using a Box-Wilson central composite design protocol. The SSF process was studied using free enzyme and free cells and it was found that with sago starch, maximum ethanol concentration of 70.68 g/l was obtained using a starch concentration of 140 g/l, which represents an ethanol yield of 97.08%. The optimum conditions for the above yield were found to be a temperature of 36.74degreesC, pH of 5.02 and time of fermentation of 17 h. Thus by using the central composite design, it is possible to determine the accurate values of the fermentation parameters where maximum production of ethanol occurs.
引用
收藏
页码：523 / 526
页数：4
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