Dried blood spot specimens for SARS-CoV-2 antibody testing: A multi-site, multi-assay comparison

被引：28

作者：

Cholette, Francois ^{[1
,2
]}

Mesa, Christine ^{[1
]}

Harris, Angela ^{[1
]}

Ellis, Hannah ^{[1
]}

Cachero, Karla ^{[1
]}

Lacap, Philip ^{[1
]}

Galipeau, Yannick ^{[3
]}

Langlois, Marc-Andre ^{[3
,4
]}

Gingras, Anne-Claude ^{[5
,6
]}

Yansouni, Cedric P. ^{[7
]}

Papenburg, Jesse ^{[7
,8
]}

Cheng, Matthew P. ^{[7
,8
]}

Chakraborty, Pranesh ^{[9
]}

Stein, Derek R. ^{[2
,10
]}

Van Caeseele, Paul ^{[2
,10
,11
,12
]}

Bartlett, Sofia ^{[13
,14
]}

Krajden, Mel ^{[13
,14
]}

Goldfarb, David ^{[14
]}

McGeer, Allison ^{[12
,14
,15
]}

Osiowy, Carla ^{[1
]}

Hankins, Catherine ^{[12
,16
]}

Mazer, Bruce ^{[17
]}

Drebot, Michael ^{[1
]}

Kim, John ^{[1
]}

机构：

[1] Publ Hlth Agcy Canada, Natl Microbiol Lab, Winnipeg, MB, Canada

[2] Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada

[3] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada

[4] Univ Ottawa, Ctr Infect Immun & Inflammat CI3, Ottawa, ON, Canada

[5] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada

[6] Mt Sinai Hosp, Sinai Hlth Syst, Lunenfeld Tanenbaum Res Inst, Toronto, ON, Canada

[7] McGill Univ, McGill Interdisciplinary Initiat Infect & Immun, Montreal, PQ, Canada

[8] McGill Univ, Hlth Ctr, Div Microbiol, Dept Clin Lab Med,Optilab Montrel, Montreal, PQ, Canada

[9] Childrens Hosp Eastern Ontario, Newborn Screening Ontario, Ottawa, ON, Canada

[10] Manitoba Hlth, Cadham Prov Lab, Winnipeg, MB, Canada

[11] Univ Manitoba, Dept Pediat & Child Hlth, Winnipeg, MB, Canada

[12] COVID 19 Immun Task Force, Montreal, PQ, Canada

[13] British Columbia Ctr Dis Control, Vancouver, BC, Canada

[14] Univ British Columbia, Dept Pathol & Lab Med, Vancouver, BC, Canada

[15] Univ Toronto, Div Infect Dis, Toronto, ON, Canada

[16] McGill Univ, Dept Epidemiol Biostat & Occupat Hlth, Montreal, PQ, Canada

[17] McGill Univ, Dept Pediat, Montreal, PQ, Canada

来源：

PLOS ONE | 2021年 / 16卷 / 12期

关键词：

D O I：

10.1371/journal.pone.0261003

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The true severity of infection due to COVID-19 is under-represented because it is based on only those who are tested. Although nucleic acid amplifications tests (NAAT) are the gold standard for COVID-19 diagnostic testing, serological assays provide better population-level SARS-CoV-2 prevalence estimates. Implementing large sero-surveys present several logistical challenges within Canada due its unique geography including rural and remote communities. Dried blood spot (DBS) sampling is a practical solution but comparative performance data on SARS-CoV-2 serological tests using DBS is currently lacking. Here we present test performance data from a well-characterized SARS-CoV-2 DBS panel sent to laboratories across Canada representing 10 commercial and 2 in-house developed tests for SARS-CoV-2 antibodies. Three commercial assays identified all positive and negative DBS correctly corresponding to a sensitivity, specificity, positive predictive value, and negative predictive value of 100% (95% CI = 72.2, 100). Two in-house assays also performed equally well. In contrast, several commercial assays could not achieve a sensitivity greater than 40% or a negative predictive value greater than 60%. Our findings represent the foundation for future validation studies on DBS specimens that will play a central role in strengthening Canada's public health policy in response to COVID-19.

引用

页数：19

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