A Global Interactome Map of the Dengue Virus NS1 Identifies Virus Restriction and Dependency Host Factors

被引：83

作者：

Hafirassou, Mohamed Lamine ^{[1
]}

Meertens, Laurent ^{[1
]}

Umana-Diaz, Claudia ^{[1
]}

Labeau, Athena ^{[1
]}

Dejarnac, Ophelie ^{[1
]}

Bonnet-Madin, Lucie ^{[1
]}

Kuemmerer, Beate M. ^{[2
]}

Delaugerre, Constance ^{[3
]}

Roingeard, Philippe ^{[4
]}

Vidalain, Pierre-Olivier ^{[5
]}

Amara, Ali ^{[1
]}

机构：

[1] Univ Paris Diderot, Sorbonne Paris Cite, Hop St Louis, Inst Univ Hematol,CNRS,UMR 7212,INSERM,U944, F-75010 Paris, France

[2] Univ Bonn, Med Ctr, Inst Virol, Bonn, Germany

[3] Hop St Louis, Dept Malad Infect, F-75010 Paris, France

[4] Univ Tours, Fac Med, INSERM, MAVIVH U966, Tours, France

[5] Univ Paris 05, Equipe Chim & Biol, Modelisat & Immunol Therapie, CNRS,UMR 8601, Paris, France

来源：

CELL REPORTS | 2017年 / 21卷 / 13期

关键词：

N-LINKED GLYCOSYLATION; TRANSLATION INITIATION; CYTOSOLIC CHAPERONIN; ENVELOPE PROTEIN; REPLICATION; INFECTION; SCREEN; RACK1;

D O I：

10.1016/j.celrep.2017.11.094

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Dengue virus (DENV) infections cause the most prevalent mosquito-borne viral disease worldwide, for which no therapies are available. DENV encodes seven non-structural (NS) proteins that co-assemble and recruit poorly characterized host factors to form the DENV replication complex essential for viral infection. Here, we provide a global proteomic analysis of the human host factors that interact with the DENV NS1 protein. Combined with a functional RNAi screen, this study reveals a comprehensive network of host cellular processes involved in DENV infection and identifies DENV host restriction and dependency factors. We highlight an important role of RACK1 and the chaperonin TRiC (CCT) and oligosaccharyltransferase (OST) complexes during DENV replication. We further show that the OST complex mediates NS1 and NS4B glycosylation, and pharmacological inhibition of its N-glycosylation function strongly impairs DENV infection. In conclusion, our study provides a global interactome of the DENV NS1 and identifies host factors targetable for antiviral therapies.

引用

收藏

页码：3900 / 3913

页数：14

相关论文

共 71 条

[1] Revisiting Dengue Virus-Host Cell Interaction: New Insights into Molecular and Cellular Virology [J].

Acosta, Eliana G. ;

Kumar, Anil ;

Bartenschlager, Ralf .

ADVANCES IN VIRUS RESEARCH, VOL 88, 2014, 88 :1-109

[2] RACK1, A multifaceted scaffolding protein: Structure and function [J].

Adams, David R. ;

Ron, Dorit ;

Kiely, Patrick A. .

CELL COMMUNICATION AND SIGNALING, 2011, 9

[3] Flavivirus NS1 Structures Reveal Surfaces for Associations with Membranes and the Immune System [J].

Akey, David L. ;

Brown, W. Clay ;

Dutta, Somnath ;

Konwerski, Jamie ;

Jose, Joyce ;

Jurkiw, Thomas J. ;

DelProposto, James ;

Ogata, Craig M. ;

Skiniotis, Georgios ;

Kuhn, Richard J. ;

Smith, Janet L. .

SCIENCE, 2014, 343 (6173) :881-885

[4] Coupling of replication and assembly in flaviviruses [J].

Apte-Sengupta, Swapna ;

Sirohi, Devika ;

Kuhn, Richard J. .

CURRENT OPINION IN VIROLOGY, 2014, 9 :134-142

[5] The global distribution and burden of dengue [J].

Bhatt, Samir ;

Gething, Peter W. ;

Brady, Oliver J. ;

Messina, Jane P. ;

Farlow, Andrew W. ;

Moyes, Catherine L. ;

Drake, John M. ;

Brownstein, John S. ;

Hoen, Anne G. ;

Sankoh, Osman ;

Myers, Monica F. ;

George, Dylan B. ;

Jaenisch, Thomas ;

Wint, G. R. William ;

Simmons, Cameron P. ;

Scott, Thomas W. ;

Farrar, Jeremy J. ;

Hay, Simon I. .

NATURE, 2013, 496 (7446) :504-507

[6] Clinical efficacy and safety of a novel tetravalent dengue vaccine in healthy children in Asia: a phase 3, randomised, observer-masked, placebo-controlled trial [J].

Capeding, Maria Rosario ;

Ngoc Huu Tran ;

Hadinegoro, Sri Rezeki S. ;

Ismail, Hussain Imam H. J. Muhammad ;

Chotpitayasunondh, Tawee ;

Chua, Mary Noreen ;

Chan Quang Luong ;

Rusmil, Kusnandi ;

Wirawan, Dewa Nyoman ;

Nallusamy, Revathy ;

Pitisuttithum, Punnee ;

Thisyakorn, Usa ;

Yoon, In-Kyu ;

van der Vliet, Diane ;

Langevin, Edith ;

Laot, Thelma ;

Hutagalung, Yanee ;

Frago, Carina ;

Boaz, Mark ;

Wartel, T. Anh ;

Tornieporth, Nadia G. ;

Saville, Melanie ;

Bouckenooghe, Alain .

LANCET, 2014, 384 (9951) :1358-1365

[7] Quantitative Proteomic Analysis of Host-virus Interactions Reveals a Role for Golgi Brefeldin A Resistance Factor 1 (GBF1) in Dengue Infection [J].

Carpp, Lindsay N. ;

Rogers, Richard S. ;

Moritz, Robert L. ;

Aitchison, John D. .

MOLECULAR & CELLULAR PROTEOMICS, 2014, 13 (11) :2836-2854

[8] A Combined Genetic-Proteomic Approach Identifies Residues within Dengue Virus NS4B Critical for Interaction with NS3 and Viral Replication [J].

Chatel-Chaix, Laurent ;

Fischl, Wolfgang ;

Scaturro, Pietro ;

Cortese, Mirko ;

Kallis, Stephanie ;

Bartenschlager, Marie ;

Fischer, Bernd ;

Bartenschlager, Ralf .

JOURNAL OF VIROLOGY, 2015, 89 (14) :7170-7186

[9] N-linked glycosylation and homeostasis of the endoplasmic reticulum [J].

Cherepanova, Natalia ;

Shrimal, Shiteshu ;

Gilmore, Reid .

CURRENT OPINION IN CELL BIOLOGY, 2016, 41 :57-65

[10] The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing [J].

De Maio, Federico A. ;

Risso, Guillermo ;

Iglesias, Nestor G. ;

Shah, Priya ;

Pozzi, Berta ;

Gebhard, Leopoldo G. ;

Mammi, Pablo ;

Mancini, Estefania ;

Yanovsky, Marcelo J. ;

Andino, Raul ;

Krogan, Nevan ;

Srebrow, Anabella ;

Gamarnik, Andrea V. .

PLOS PATHOGENS, 2016, 12 (08)

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