Aptamer based photoelectrochemical cytosensor with layer-by-layer assembly of CdSe semiconductor nanoparticles as photoelectrochemically active species

被引：99

作者：

Zhang, Xiaoru ^{[1
]}

Li, Shuguo ^{[1
]}

Jin, Xia ^{[1
]}

Li, Xuemei ^{[1
]}

机构：

[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, State Key Lab Base Ecochem Engn, Qingdao 266042, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 08期

关键词：

Photoelectrochemistry; Cytosensor; Aptamer; CdSe semiconductor nanoparticles; CELL-SURFACE CARBOHYDRATE; CIRCULATING TUMOR-CELLS; RAPID DETECTION; FENTON REACTION; QUANTUM DOTS; DNA; DIAGNOSTICS; ELECTRODE; LIGANDS; SENSOR;

D O I：

10.1016/j.bios.2011.01.030

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A label-free photoelectrochemical cytosensor for highly sensitive and specific detection of Ramos cell was developed based on photoactive films. The films were fabricated by a layer-by-layer (LBL) assembly of positively charged poly(dimethyldiallylammonium chloride) (PDDA) and negatively charged CdSe semiconductor nanoparticles (NPs) capped with mercaptoacetic acid. The resulting modified electrodes were tested as sensors for Ramos cell through the recognition of DNA aptamer which was covalently bound to the electrode using the classic coupling reactions between -COOH groups on the surfaces of CdSe NPs and -NH2 groups of the aptamer. The newly developed cytosensor exhibited excellent sensitivity and selectivity. The linear range was from 160 to 1600 cells/mL and the detection limit was 84 cells/mL. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：3674 / 3678

页数：5

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