Early Regional Response of Apoptotic Activity in Newborn Piglet Brain Following Hypoxia and Ischemia

被引：9

作者：

Pirzadeh, A. ^{[2
]}

Mammen, A. ^{[2
]}

Kubin, J. ^{[1
]}

Reade, E. ^{[2
]}

Liu, H. ^{[1
]}

Mendoza, A. ^{[1
]}

Greeley, W. J. ^{[2
]}

Wilson, D. F. ^{[1
]}

Pastuszko, A. ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

[2] Childrens Hosp Philadelphia, Dept Anesthesiol & Crit Care Med, Philadelphia, PA 19104 USA

来源：

NEUROCHEMICAL RESEARCH | 2011年 / 36卷 / 01期

关键词：

Brain; Apoptosis; Hypoxia; Ischemia; Newborn; PROGRAMMED CELL-DEATH; MIDDLE CEREBRAL-ARTERY; CREB PHOSPHORYLATION; NERVOUS-SYSTEM; IN-VIVO; DNA FRAGMENTATION; UP-REGULATION; RAT-BRAIN; BCL-2; NEURONS;

D O I：

10.1007/s11064-010-0267-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Responses of selected neuroregulatory proteins that promote (Caspase 3 and Bax) or inhibit (Bcl-2, high Bcl-2/Bax ratio) apoptotic cell death were measured in the brain of piglets subjected to precisely controlled hypoxic and ischemic insults: 1 h hypoxia (decreasing FiO(2) from 21 to 6%) or ischemia (ligation of carotid arteries and hemorrhage), followed by 0, 2 and 4 h recovery with 21% FiO(2). Protein expression was measured in cortex, hippocampus and striatum by Western blot. There were no significant differences in expression of Caspase-3 between sham operated, hypoxic and ischemic groups. There were significant regional differences in expression of Bcl-2 and Bax in response to hypoxia and ischemia. The changes in Bcl-2/Bax ratio were similar for hypoxia and ischemia except for striatum at zero time recovery, with ischemia giving lower ratios than hypoxia. The Bcl-2/Bax ratio was also lower for the striatum than for the other regions of the brain, suggesting this region is the more susceptible to apoptotic injury.

引用

页码：83 / 92

页数：10

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