Alpha-Amylase and Alpha-Glucosidase Enzyme Inhibition and Antioxidant Potential of 3-Oxolupenal and Katononic Acid Isolated from Nuxia oppositifolia

被引:176
作者
Alqahtani, Ali S. [1 ,2 ]
Hidayathulla, Syed [1 ]
Rehman, Md Tabish [2 ]
ElGamal, Ali A. [2 ]
Al-Massarani, Shaza [2 ]
Razmovski-Naumovski, Valentina [3 ]
Alqahtani, Mohammed S. [4 ]
El Dib, Rabab A. [5 ]
AlAjmi, Mohamed F. [2 ]
机构
[1] King Saud Univ, Coll Pharm, Med Aromat & Poisonous Plants Res Ctr MAPRC, POB 2457, Riyadh 11451, Saudi Arabia
[2] King Saud Univ, Coll Pharm, Dept Pharmacognosy, POB 2457, Riyadh 11451, Saudi Arabia
[3] Univ New South Wales, Sch Med, South Western Sydney Clin Sch, Sydney, NSW 2052, Australia
[4] King Saud Univ, Coll Pharm, Dept Pharmaceut, Riyadh 11451, Saudi Arabia
[5] Helwan Univ, Fac Pharm, Dept Pharmacognosy, Cairo 11795, Egypt
关键词
Nuxia oppositifolia; antioxidant; 3-oxolupenal; katononic acid; DPPH; ABTS; glucosidase; amylase; docking; HUMAN SERUM-ALBUMIN; URSOLIC ACID; HEXANE FRACTION; TGR5; AGONIST; EXTRACT;
D O I
10.3390/biom10010061
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nuxia oppositifolia is traditionally used in diabetes treatment in many Arabian countries; however, scientific evidence is lacking. Hence, the present study explored the antidiabetic and antioxidant activities of the plant extracts and their purified compounds. The methanolic crude extract of N. oppositifolia was partitioned using a two-solvent system. The n-hexane fraction was purified by silica gel column chromatography to yield several compounds including katononic acid and 3-oxolupenal. Antidiabetic activities were assessed by alpha-amylase and alpha-glucosidase enzyme inhibition. Antioxidant capacities were examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2 '-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) scavenging assays. Further, the interaction between enzymes (alpha-amylase and alpha-glucosidase) and ligands (3-oxolupenal and katononic acid) was followed by fluorescence quenching and molecular docking studies. 3-oxolupenal and katononic acid showed IC50 values of 46.2 mu g/mL (101.6 mu M) and 52.4 mu g/mL (119.3 mu M), respectively against the amylase inhibition. 3-oxolupenal (62.3 mu g/mL or 141.9 mu M) exhibited more potent inhibition against alpha-glucosidases compared to katononic acid (88.6 mu g/mL or 194.8 mu M). In terms of antioxidant activity, the relatively polar crude extract and n-butanol fraction showed the greatest DPPH and ABTS scavenging activity. However, the antioxidant activities of the purified compounds were in the low to moderate range. Molecular docking studies confirmed that 3-oxolupenal and katononic acid interacted strongly with the active site residues of both alpha-amylase and alpha-glucosidase. Fluorescence quenching results also suggest that 3-oxolupenal and katononic acid have a good affinity towards both alpha-amylase and alpha-glucosidase enzymes. This study provides preliminary data for the plant's use in the treatment of type 2 diabetes mellitus.
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页数:19
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