Fasciola hepatica: Comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats

被引：25

作者：

Duthaler, Urs ^{[1
,2
]}

Rinaldi, Laura ^{[3
]}

Maurelli, Maria P. ^{[3
]}

Vargas, Mireille ^{[1
,2
]}

Utzinger, Juerg ^{[2
,4
]}

Cringoli, Giuseppe ^{[3
]}

Keiser, Jennifer ^{[1
,2
]}

机构：

[1] Swiss Trop & Publ Hlth Inst, Dept Med Parasitol & Infect Biol, CH-4002 Basel, Switzerland

[2] Univ Basel, CH-4003 Basel, Switzerland

[3] Univ Naples Federico II, Dept Pathol & Anim Hlth, CREMOPAR Reg Campania, I-80137 Naples, Italy

[4] Swiss Trop & Publ Hlth Inst, Dept Epidemiol & Publ Hlth, CH-4002 Basel, Switzerland

来源：

EXPERIMENTAL PARASITOLOGY | 2010年 / 126卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

Fasciola hepatica; Diagnosis; Sedimentation; FLOTAC; Sensitivity; Faecal egg count; Efficiency; ZOONOTIC HELMINTH INFECTIONS; COPROMICROSCOPIC DIAGNOSIS; MCMASTER; EFFICACY;

D O I：

10.1016/j.exppara.2010.04.020

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We compared the sedimentation and FLOTAC techniques for the detection and quantification of Fasciola hepatica eggs in faecal samples obtained from 120 experimentally-infected rats before intervention, and in 42 rats after drug administration. Additionally, the average time for a single test was determined. A single FLOTAC showed a higher sensitivity (92.6%) than 2, 4 and 8 sedimentation readings (63.0-85.2%) for detecting F. hepatica eggs in rat faeces post-treatment. On average, it took 21 min to prepare and examine a single FLOTAC, whereas 114 min were needed for the sedimentation method including the reading of 8 slides. In both treated and untreated rats, the sedimentation method resulted in higher mean faecal egg counts (FECs) than FLOTAC (P < 0.05). In view of the high sensitivity and efficiency, the FLOTAC technique holds promise for experimental work in the F. hepatica-rat model. Additional research is needed to determine the reasons for the observed differences in FECs. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：161 / 166

页数：6

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