An Extract of Antrodia camphorata Mycelia Attenuates the Progression of Nephritis in Systemic Lupus Erythematosus-Prone NZB/W F1Mice

被引:22
作者
Chang, Jia-Ming [1 ]
Lee, Yi-Ru [2 ]
Hung, Le-Mei [1 ]
Liu, Sheng-Yung [3 ]
Kuo, Mao-Tien [3 ]
Wen, Wu-Che [3 ]
Chen, Peini [4 ]
机构
[1] Dev Ctr Biotechnol, Div Res & Dev, Xizhi City 221, Taipei County, Taiwan
[2] Natl Taiwan Univ, Inst Mol Biol & Biochem, Coll Med, Taipei 111, Taiwan
[3] Golden Biotechnol Corp, Danshuei Township 251, Taipei County, Taiwan
[4] Taipei Vet Gen Hosp, Dept Radiol, Taipei 112, Taiwan
关键词
TUMOR-NECROSIS-FACTOR; FRUITING BODIES; GENE-EXPRESSION; DISEASE; MOUSE; MODEL; MICE;
D O I
10.1093/ecam/nen057
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Antrodia camphorata is used in folk medicine for the treatment of inflammation syndromes and liver-related diseases in Taiwan. The goal of this study was to evaluate the efficacy of the mycelial extract of A. camphorata (ACE) for the treatment of systemic lupus erythematosus (SLE) in SLE-prone NZB/W F1 mice. After antibodies against double-stranded DNA appeared in NZB/W mice, the mice were orally administered varying dosages of ACE (100, 200 and 400 mg kg(-1)) for 5 consecutive days per week for 12 weeks via gavage. To assess the efficacy of ACE, we measured SLE-associated biochemical and histopathological biomarkers levels of blood urine nitrogen (BUN), blood creatinine, urine protein and urine creatinine and thickness of the kidney glomerular basement membrane by staining with periodic acid-Schiff. Antroquinonol, an active component of ACE, was investigated for anti-inflammation activity in lipopolysaccharide-induced RAW 267.4 cells. ACE at 400 mg dkg(-1) significantly suppressed urine protein and serum BUN levels and decreased the thickness of the kidney glomerular basement membrane. Antroquinonol significantly inhibited the production of tumor necrosis factor-alpha and interleukin-1 beta by 75 and 78%, respectively. In conclusion, ACE reduced urine protein and creatinine levels and suppressed the thickening of the kidney glomerular basement membrane, suggesting that ACE protects the kidney from immunological damage resulting from autoimmune disease.
引用
收藏
页码:1 / 7
页数:7
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