Study of the interaction between N-confused porphyrin and bovine serum albumin by fluorescence spectroscopy

被引：51

作者：

Yu, Xianyong ^{[1
]}

Liu, Ronghua ^{[1
]}

Yi, Rongqiong ^{[1
]}

Yang, Fengxian ^{[1
]}

Huang, Haowen ^{[1
]}

Chen, Jian ^{[1
]}

Ji, Danhong ^{[1
]}

Yang, Ying ^{[1
]}

Li, Xiaofang ^{[1
]}

Yi, Pinggui ^{[1
]}

机构：

[1] Hunan Univ Sci & Technol, Key Lab Theoret Chem & Mol Simulat, Sch Chem & Chem Engn, Minist Educ,Hunan Prov Coll,Key Lab QSAR QSPR, Xiangtan 411201, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2011年 / 78卷 / 04期

基金：

中国国家自然科学基金;

关键词：

N-confused porphyrin; Bovine serum albumin; Fluorescence spectroscopy; Absorption spectroscopy; Interaction; BINDING INTERACTION; MECHANISM; ACID; HYDROCHLORIDE; FLAVONOIDS; SERIES; ISOMER;

D O I：

10.1016/j.saa.2011.01.024

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The fluorescence and ultraviolet spectroscopy were explored to study the interaction between N-confused porphyrins (NCP) and bovine serum albumin (BSA) under imitated physiological condition. The experimental results indicated that the fluorescence quenching mechanism between BSA and NCR was static quenching procedure at low NCR concentration at 293 and 305 K or a combined quenching (static and dynamic) procedure at higher NCP concentration at 305 K. The binding constants, binding sites and the corresponding thermodynamic parameters Delta H, Delta S, and Delta G were calculated at different temperatures. The comparison of binding potency of the three NCR to BSA showed that the substituting groups in benzene ring could enhance the binding affinity. From the thermodynamic parameters, we concluded that the action force was mainly hydrophobic interaction. The binding distances between NCP and BSA were calculated using Forster non-radiation energy transfer theory. In addition, the effect of NCP on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1329 / 1335

页数：7

共 42 条

[1] PHOTOSENSITIZERS OF THE PORPHYRIN AND PHTHALOCYANINE SERIES FOR PHOTODYNAMIC THERAPY [J].