Accurate rotation of ultra-thin glass chamber for single-cell multidirectional observation

We report an on-chip single-cell multidirectional observation method based on accurately controlling the ultra-thin glass chamber rotation angles over a microscope. This technique enables one to establish multidirectional visual access to a target cell captured inside of the thin glass chamber. The ultra-thin glass chamber has the advantages of being highly transparent, compact, thin-walled, and less invasive and having an all-closed structure, which efficiently reduces the risk of contamination during observation. The fabricated thin glass chambers' diameters varied from 1.5 to 3.5 mm which were formed on 200 mu m glass slides.