Suppression of macrophage infiltration into the conjunctiva by clodronate liposomes in experimental immune-mediated blepharoconjunctivitis

被引：11

作者：

Fukushima, A ^{[1
]}

Ozaki, A

Ishida, W

van Rooijen, N

Fukata, K

Uena, H

机构：

[1] Kochi Med Sch, Dept Ophthalmol, Immunol Lab, Nankoku, Kochi 7838505, Japan

[2] Vrije Univ Amsterdam, Fac Med, Dept Cell Biol & Immunol, Amsterdam, Netherlands

来源：

CELL BIOLOGY INTERNATIONAL | 2005年 / 29卷 / 04期

关键词：

clodronate liposomes; ED1; ED2; experimental immune-mediated blepharoconjunctivitis; macrophage;

D O I：

10.1016/j.cellbi.2004.12.011

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Macrophages infiltrate the conjunctiva in severe cases of allergic conjunctivitis (AC) such as atopic keratoconjunctivitis (AKC). We established experimental immune-mediated blepharoconjunctivitis (EC) in Brown Norway (BN) rats as a model for severe types of AC. We investigated whether macrophage infiltration in the conjunctiva in this EC model is inhibited by clodronate liposomes (CL2MDP-lip). The numbers of ED1-positive but not ED2-positive macrophages in the conjunctivas were increased by the induction of EC. Subconjunctival injection of CL2MDP-lip decreased the number of ED2-positive but not ED1-positive macrophages in the conjunctivas of naive rats. CL2MDP-lip did not affect macrophages in the spleen. Subconjunctival injection of CL2MDP-lip into EC-developing BN rats decreased the number of ED2-positive macrophages at all the time points. ED1-positive cell infiltration was inhibited when treatment was administered just prior to OVA challenge. Intravenous injection of CL2MDP-lip decreased the number of ED2-positive cells in the conjunctiva. Thus, we conclude that CL2MDP-lip inhibits infiltration of macrophages into the conjunctiva within 24 h of antigen challenge. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：277 / 286

页数：10

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