Identification of Dinitrotoluene Selective Peptides by Phage Display Cloning

被引：12

作者：

Jong, Hyeon-Jun ^{[1
]}

Na, Jung-Hyun ^{[1
]}

Jin, Bong-Suk ^{[1
]}

Lee, Won-Kyu ^{[1
]}

Lee, Woong-Hee ^{[1
]}

Jung, Hyun Jin ^{[2
]}

Kim, Seok-Chan ^{[1
]}

Lim, Si-Hyung ^{[3
]}

Yu, Yeon Gyu ^{[1
]}

机构：

[1] Kookmin Univ, Dept Chem, Seoul 136702, South Korea

[2] Kookmin Univ, Dept Mech & Design, Seoul 136702, South Korea

[3] Kookmin Univ, Sch Mech Engn, Seoul 136702, South Korea

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2010年 / 31卷 / 12期

关键词：

Explosive; DNT; Sensor; Peptide; Phage display; EXPLOSIVES; TNT;

D O I：

10.5012/bkcs.2010.31.12.3703

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Biomolecules specific to explosives can be exploited as chemical sensors. Peptides specific to immobilized dinitrotoluene (DNT) were identified using a phage display library. A derivative of DNT that contained an extended amine group, 4-(2,4-dinitrophenyl)butan-1-amine, was synthesized and immobilized using a self-assembled monolayer surface on gold. Filamentous M13 phages displaying random sequences of 12-mer peptides specific to the immobilized DNT-derivate were isolated from the M13 phage library by biopanning. A common peptide sequence was identified from the isolated phages and the synthesized peptides showed selective binding to DNT. When the peptide was immobilized on a quartz crystal microbalance (QCM) chip, it showed a-binding signal to DNT, while toluene barely showed significant binding to the QCM chip. These results demonstrate that peptides screened by biopanning against immobilized DNT can be useful for quick and accurate detection of DNT.

引用

页码：3703 / 3706

页数：4

共 13 条

[1] Use of reversed-phase high-performance liquid chromatography-diode array detection for complete separation of 2,4,6-trinitrotoluene metabolites and EPA method 8330 explosives: influence of temperature and an ion-pair reagent [J].