Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software

被引：0
作者：
Ledesma, Alba [1 ,2 ]
Zalazar, Lucia [2 ,3 ]
Greco, Micaela [2 ,3 ]
Hozbor, Federico [1 ]
Cesari, Andreina [2 ,3 ]
机构：
[1] Inst Nacl Tecnol Agr INTA, Biotecnol Reprod, Ruta 226 Km 73-5, RA-7620 Balcarce, Argentina
[2] Consejo Nacl Invest Cient & Tecn CONICET, Buenos Aires, DF, Argentina
[3] Univ Nacl Mar del Plata, FCEyN, Biol Microorganismos & Gametas, Inst Invest Biol CONICET, Funes 3250, RA-7600 Mar Del Plata, Argentina
来源：
TROPICAL ANIMAL HEALTH AND PRODUCTION | 2021年 / 53卷 / 05期
关键词：
Sperm subpopulations; Recombinant proteins; Semen extender; Ram; SEMINAL PLASMA-PROTEINS; MAMMALIAN SPERMATOZOA; CRYOPRESERVATION; FERTILIZATION; MEMBRANE; SEMEN; SUBPOPULATIONS; CRYODAMAGE; FERTILITY; CAPACITY;
D O I：
10.1007/s11250-021-02954-2
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His(6,) a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 mu M and 3.0 mu M of TrxAFNIIx4His(6). Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His(6) improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His(6) increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His(6) to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.
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页数：6
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