The PCK-PCR clonality assay (PPCA)

被引：4
作者：
Busque, L
Gilliland, DG
机构：
[1] HOP MAISON NEUVE ROSEMONT,HAEMATOL & IMMUNOL CTR,MONTREAL,PQ H1T 2M4,CANADA
[2] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,BOSTON,MA 02115
来源：
MOLECULAR BIOTECHNOLOGY | 1996年 / 5卷 / 03期
关键词：
clonality; polymerase chain reaction; phosphoglycerate kinase gene; X chromosome inactivation;
D O I：
10.1007/BF02900366
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The PGK-PCR assay offers an accurate method for determination of clonal derivation of cells in females who are heterozygous for the PGK BstXI polymorphism. Techniques to screen for heterozygosity and determine clonality using polymerase chain reaction based techniques are provided.
引用
收藏
页码：275 / 280
页数：6
相关论文
共 11 条
[1]   PRIMORDIAL CELL POOL SIZE AND LINEAGE RELATIONSHIPS OF 5 HUMAN CELL TYPES [J].
FIALKOW, PJ .
ANNALS OF HUMAN GENETICS, 1973, 37 (JUL) :39-48
[2]   X-CHROMOSOME INACTIVATION PATTERNS USING HPRT AND PGK POLYMORPHISMS IN HEMATOLOGICALLY NORMAL AND POSTCHEMOTHERAPY FEMALES [J].
GALE, RE ;
WHEADON, H ;
LINCH, DC .
BRITISH JOURNAL OF HAEMATOLOGY, 1991, 79 (02) :193-197
[3]   CLONALITY IN MYELOPROLIFERATIVE DISORDERS - ANALYSIS BY MEANS OF THE POLYMERASE CHAIN-REACTION [J].
GILLILAND, DG ;
BLANCHARD, KL ;
LEVY, J ;
PERRIN, S ;
BUNN, HF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (15) :6848-6852
[4]  
GILLILAND G, 1989, CLIN RES, V37, pA601
[5]  
GRANT SG, 1988, ANNU REV GENET, V22, P199, DOI 10.1146/annurev.ge.22.120188.001215
[6]  
LYON MF, 1988, AM J HUM GENET, V42, P8
[7]   INVIVO FOOTPRINT AND METHYLATION ANALYSIS BY PCR-AIDED GENOMIC SEQUENCING - COMPARISON OF ACTIVE AND INACTIVE X-CHROMOSOMAL DNA AT THE CPG ISLAND AND PROMOTER OF HUMAN PGK-1 [J].
PFEIFER, GP ;
TANGUAY, RL ;
STEIGERWALD, SD ;
RIGGS, AD .
GENES & DEVELOPMENT, 1990, 4 (08) :1277-1287
[8]   THE USE OF CELL MARKERS IN THE STUDY OF HUMAN HEMATOPOIETIC NEOPLASIA [J].
RASKIND, WH ;
FIALKOW, PJ .
ADVANCES IN CANCER RESEARCH, 1987, 49 :127-167
[9]   X-CHROMOSOME INACTIVATION AND CELL MEMORY [J].
RIGGS, AD ;
PFEIFER, GP .
TRENDS IN GENETICS, 1992, 8 (05) :169-174
[10]   USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS TO DETERMINE THE CLONAL ORIGIN OF HUMAN-TUMORS [J].
VOGELSTEIN, B ;
FEARON, ER ;
HAMILTON, SR ;
FEINBERG, AP .
SCIENCE, 1985, 227 (4687) :642-645
12