Analysis of sofalcone in human plasma by high performance liquid chromatography

被引：17

作者：

Aidong, Wen ^{[1
]}

Zhipeng, Wang

Taijun, Hang

Yanyan, Jia

Tingting, Zhang

Yin, Wu

Xuan, Gao

Zhifu, Yang

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Pharm, Xian, Peoples R China

[2] Fourth Mil Med Univ, Sch Pharm, Dept Pharmacol, Xian, Peoples R China

[3] China Pharmaceut Univ, Dept Pharmaceut Anal, Nanjing, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 856卷 / 1-2期

关键词：

sofalcone; high performance liquid chromatography; plasma;

D O I：

10.1016/j.jchromb.2007.05.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid, sensitive and reliable high performance liquid chromatography (HPLC) method for the determination of the anti-ulcer drug sofalcone in human plasma was developed. Plasma was extracted with ethyl acetate under acidic conditions and sofalcone was determined by HPLC using a C 18 column and (methanol-0.1% formic acid aqueous 80:20) mobile phase. The linear calibration curves of sofalcone in human plasma were obtained over the concentration range of 0.01-5.0 mu g/ml. The lower limit of quantitation (LLOQ) was 10 ng/ml in human plasma. The precision measured for plasma was within 15%. Extraction recovery was over 85% in blood. The method was successfully applied to the identification and quantification of sofalcone in pharmacokinetic studies. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：348 / 352

页数：5

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