Immobilization of Papain onto Graphene Oxide Nanosheets

被引：17

作者：

Gu, Xiangyu ^{[1
]}

Gao, Jing ^{[2
]}

Li, Xiaohan ^{[2
]}

Wang, Yue ^{[3
]}

机构：

[1] Jiangsu Univ Sci & Technol, Sch Grain Sci & Technol, Zhenjiang 212009, Peoples R China

[2] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China

[3] Guangxi Med Univ, Sch Pharm, Nanning 530021, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2018年 / 18卷 / 05期

关键词：

Graphene Oxide; Papain; Modification; Immobilization; Stability; COVALENT IMMOBILIZATION; MAGNETIC NANOPARTICLES; FUNCTIONALIZATION; LIPASE; NANOCOMPOSITES; FE3O4-AT-SIO2; COMPOSITE;

D O I：

10.1166/jnn.2018.14695

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene oxide nanosheets was prepared, functionlized with 3-aminopropyltriethoxysilane and used as the carrier for immobilization of papain. A high immobilization yield and efficiency higher than 80% were achieved at pH 8.0 and 35 degrees C. The thermal stability of the immobilized papain was improved compared with the free enzyme. And the immobilized papain retained about 63% of its initial activity after 30 days storage at 4 degrees C, while free enzyme was only 41% at the same conditions. The immobilized enzyme displayed higher binding affinity to substrate because of the unique properties of GO. These results indicate that modified graphene oxide nanosheets has a good potential as carrier for immobilization of proteins, especially enzymes.

引用

页码：3543 / 3547

页数：5

共 29 条

[1] Recent developments on graphene and graphene oxide based solid state gas sensors [J].

Basu, S. ;

Bhattacharyya, P. .

SENSORS AND ACTUATORS B-CHEMICAL, 2012, 173 :1-21

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] A novel cross-linked enzyme aggregates (CLEAs) of papain and neutrase-production, partial characterization and application [J].

Chen, Zhongqin ;

Wang, Yanwei ;

Liu, Wei ;

Wang, Jingya ;

Chen, Haixia .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 95 :650-657

[4] Papain from papaya (Carica papaya L.) fruit and latex: Preliminary characterization in alcoholic-acidic buffer for wine application [J].

Esti, Marco ;

Benucci, Ilaria ;

Lombardelli, Claudio ;

Liburdi, Katia ;

Garzillo, Anna Maria Vittoria .

FOOD AND BIOPRODUCTS PROCESSING, 2013, 91 (C4) :595-598

[5] Noncovalent Functionalization of Graphene and Graphene Oxide for Energy Materials, Biosensing, Catalytic, and Biomedical Applications [J].

Georgakilas, Vasilios ;

Tiwari, Jitendra N. ;

Kemp, K. Christian ;

Perman, Jason A. ;

Bourlinos, Athanasios B. ;

Kim, Kwang S. ;

Zboril, Radek .

CHEMICAL REVIEWS, 2016, 116 (09) :5464-5519

[6] Graphene-based composites [J].

Huang, Xiao ;

Qi, Xiaoying ;

Boey, Freddy ;

Zhang, Hua .

CHEMICAL SOCIETY REVIEWS, 2012, 41 (02) :666-686

[7] Immobilization of dehydrogenase onto epoxy-functionalized nanoparticles for synthesis of (R)-mandelic acid [J].

Jiang, Xiao-Ping ;

Lu, Ting-Ting ;

Liu, Cai-Hong ;

Ling, Xiao-Ming ;

Zhuang, Meng-Yao ;

Zhang, Jiu-Xun ;

Zhang, Ye-Wang .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2016, 88 :9-17

[8] Chemical functionalization of graphene oxide for improving mechanical and thermal properties of polyurethane composites [J].

Jing, Qifei ;

Liu, Wanshuang ;

Pan, Yongzheng ;

Silberschmidt, Vadim V. ;

Li, Lin ;

Dong, Zhili .

MATERIALS & DESIGN, 2015, 85 :808-814

[9] The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres [J].

Lei, H ;

Wang, W ;

Chen, LL ;

Li, XC ;

Yi, B ;

Deng, L .

ENZYME AND MICROBIAL TECHNOLOGY, 2004, 35 (01) :15-21

[10] Fabrication of graphene oxide decorated with Fe3O4@SiO2 for immobilization of cellulase [J].

Li, Yue ;

Wang, Xiang-Yu ;

Jiang, Xiao-Ping ;

Ye, Jing-Jing ;

Zhang, Ye-Wang ;

Zhang, Xiao-Yun .

JOURNAL OF NANOPARTICLE RESEARCH, 2015, 17 (01)

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